在CD4和CD8的胞内结构域上附着结合着Src酪氨酸激酶家族的成员—— Lck。 当TCR和多肽-MHC复合物特异性结合后，CD4和CD8也随后和MHC分子的发生结合，这导致共受体在物理上靠近TCR复合物。 与此同时在T细胞内，CD4和CD8上的Lck分子就和CD3和ξ上的ITAM靠近，随后Lck就将ITAM上的酪氨酸残基磷酸化，ITAM的磷酸化随后导致 ZAP-70 酪氨酸激酶的募集和活化，ITAM的结构中有两个酪氨酸残基，这两个酪氨酸残基被磷酸化以后可以和ZAP-70结合，结 …

2023年1月3日 · In thymocytes and mature T cells, wild-type Lck is associated with CD4 and/or CD8 via cysteine (C) residues. Phosphorylation of Tyr505 (Y505) of Lck enables the C terminus to bind to the SH2...

The Lck is a member of Src kinase family (SKF) and is important for the activation of T-cell receptor (TCR) signaling in both naive T cells and effector T cells. The role of Lck is less prominent in the activation or in the maintenance of memory CD8 T …

2003年9月19日 · We examined the folding and solution structures of ternary CD4-Lck-Zn 2+ and CD8α-Lck-Zn 2+ complexes. The coreceptor tails and the Lck N-terminus are unstructured in isolation but assemble in the presence of zinc to form compactly folded heterodimeric domains.

2022年12月23日 · Overall, our findings reveal the role of co-receptor-bound LCK in T cell biology, show that CD4- and CD8-bound LCK drive T cell development and effector immune responses using qualitatively...

2002年2月4日 · Instead, the T cell accessory molecules CD4 and CD28 cooperated to induce autophosphorylation of Lck. CD4 recruited Lck to the T cell–APC interface, whereas CD28 sustained Lck activation....

CD4/CD8-p56 lck regulated events in T-cells include intracellular calcium mobilization, integrin activation and the induction of transcription factors for gene expression.

Using in vitro assays and noninvasive fluorescence cross-correlation spectroscopy, we show that most coreceptors are occupied by Lck at the surface of live cells. The structural basis for important differences in the kinase occupancy of CD4 and CD8αβ is also identified.

2020年2月4日 · Our mathematical model and experimental data show that the dynamic regulation of CD4- and CD8-LCK coupling establish the self-reactivity of the peripheral T cell pool. The stoichiometry of the interaction between CD8 and LCK, but not between CD4 and LCK, substantially increases upon T cell maturation.

2000年3月1日 · Since the tyrosine kinase Lck binds with more affinity to CD4 than CD8, it has been proposed as a candidate to mediate this process. By using transgenic mice with altered Lck activity, we show that thymocytes carrying a class II–restricted TCR develop into functional CD8 T cells when Lck activity is reduced.