traf3ip3向高尔基复合体募集mapk / erk激酶（mek），促进其通过braf激活并导致细胞外信号调节激酶（erk）激活。 除了一些特殊的磷酸酶（如CD45和SHP2），大多数在细胞信号传导中主要起抑制作用，直接或间接抑制蛋白激酶活性。

2000年9月15日 · Linker for T-cell activation (LAT) is a recently identified 36- to 38-kd membrane adapter protein expressed in T cells, natural killer (NK) cells, mast cells, and megakaryocytes, but not in B cells or monocytes. 10, 11 Following ligation of the TCR-CD3 complex, ZAP70 or Syk kinase mediates the tyrosine phosphorylation of LAT that, in turn, is re...

2012年10月26日 · Bam32 overexpression in Jurkat T cells deficient for LAT (JCam2.5) resulted in a robust increase in Mek and Erk activation, demonstrating that the Bam32-PLC-γ1-Pak1 pathway is independent of LAT (Figure 4A). Next we investigated the relationship of LAT-PLC-γ1 and Bam32-PLC-γ1.

2018年6月18日 · We report the discovery of a conserved proline-rich motif in LAT that mediates efficient LAT phosphorylation. Lck associates with this motif via its SH3 domain, and with phospho-Zap70 via its...

2003年11月17日 · The effects of basal phosphorylation of LAT are twofold: (1) recruitment and activation of PLCγ generates low levels of second messengers that signal through calcium and Ras pathways, culminating in Erk kinase activity; and (2) phosphorylation of tyrosine residue 6 of LAT, likely establishing a LAT–PLCγ–Abl complex, is required for low ...

2016年4月7日 · By manipulating clustering using LAT phosphorylation mutants, we show that clustering occurs through analogous mechanisms in vitro and in cells and that clustering promotes MAPK(ERK) signaling. Thus, as in three-dimensional phase separation ( 17 , 26 ), our results demonstrate that phase separation on membranes can create an environment that ...

2012年10月10日 · The indispensable function of LAT (linker for activation of T cells) in TCR (T cell receptor)-mediated signaling has been clearly demonstrated. LAT-deficient Jurkat cells have severely impaired TCR-mediated calcium mobilization, Erk activation, CD69 upregulation, and AP-1- and NFAT-mediated gene transcription .

2020年4月24日 · We construct a mathematical model to explore CAR-mediated MAPK signaling. Model predicts how intracellular CAR domains affect ERK response time. CD28 influences ERK activation by enhancing the phosphorylation kinetics of CD3ζ. Removing ITAMs from CD3ζ speeds up ERK response and reduces negative feedback.

2001年8月1日 · In this study, we have attempted to identify the critical tyrosine residues in LAT that mediate TCR activation-induced mobilization of intracellular Ca2+ and activation of the MAP kinase Erk2. By using the LAT-deficient Jurkat derivative, J.CaM2, stable cell lines were established expressing various tyrosine mutants of LAT.

2012年10月26日 · We characterized in Jurkat cells and primary T lymphocytes a pathway dependent on Bam32-PLC-γ1-Pak1 complexes, in which Pak1 kinase activates Raf-1 and Mek-1, both upstream of Erk. In the Bam32-PLC-γ1-Pak1 complex, catalytically inactive PLC-γ1 is used as a scaffold linking Bam32 to Pak1.