2014年3月2日 · Malissen and colleagues use quantitative proteomics to identify a Lat-independent TCR–CD6–SLP-76–Zap70 signalosome after TCR stimulation.

To determine the respective contribution of the LAT transmembrane adaptor and CD5 and CD6 transmembrane receptors to early TCR signal propagation, diversification, and termination, we describe a CRISPR/Cas9–based platform that uses primary mouse T cells and permits establishment of the composition of their LAT, CD5, and CD6 signalosomes in ...

2024年5月24日 · A principal component transcriptomic analysis of resting and activated naïve CD4 + T cells from mice deficient in CD5 (inhibitory scaffold), LAT (co-stimulatory scaffold), and CD6, showed that TCR- and CD28-activated Lat −/− T cells clustered with non-activated WT T cells, whereas activated Cd5 −/− and Cd6 −/− T cells clustered ...

2018年6月18日 · We report the discovery of a conserved proline-rich motif in LAT that mediates efficient LAT phosphorylation. Lck associates with this motif via its SH3 domain, and with phospho-Zap70 via its...

2018年12月17日 · Unlike PAG or LAT, CD6 contains structured ectodomains that are suitable to establish interactions with extracellular ligands. CD6 binds to CD166, widely expressed in many cell types and tissues (53, 54). and recently CD318 was identified as an alternative ligand in cells derived from human thymus, skin, synovium, and cartilage (55, 56).

2022年11月21日 · We show that deletion of the cytosolic tail of CD6 enhances T-cell responses, indicating that CD6 restrains T-cell activation. One component of the CD6-associated inhibitory apparatus was found to be the GTPase activating protein of Ras (RasGAP), that we show to associate with CD6 in a phosphorylation-dependent manner.

CD6 has an extraordinarily long cytoplasmic tail (244 amino acids) and provides an alternative to LAT as a scaffold for assemblies of signaling proteins in T cells (Roncagalli et al., 2014). In contrast, CD166 has a short cytoplasmic region that regulates adhesion through a link with the cytoskeleton ( Te Riet et al., 2014 ).

2024年5月24日 · Our study identifies CD6 as a novel 'on/off' scaffold-receptor capable of modulating responsiveness in two ways. Firstly, and independently of ligand binding, it establishes signaling thresholds through tonic inhibition, functioning as a membrane-bound scaffold. Secondly, CD6 has the capacity for al …

2021年2月1日 · To determine the respective contribution of the LAT transmembrane adaptor and CD5 and CD6 transmembrane receptors to early TCR signal propagation, diversification, and termination, we describe a CRISPR/Cas9-based platform that uses primary mouse T cells and permits establishment of the composition of their LAT, CD5, and CD6 signalosomes in only ...

The surface receptor CD6 was able to initiate its own signaling pathway by recruiting SLP-76 and the guanine nucleotide-exchange factor Vav1 regardless of the presence of Lat. Our findings provide a more complete model of TCR signaling in which CD6 constitutes a signaling hub that contributes to the diversification of TCR signaling.