2020年12月21日 · Azilsartan prevented LPS-induced expression of cyclooxygenase 2 (COX-2) and secretion of prostaglandin E2 (PGE 2) in U937 macrophages. Cells were treated with 1 μg/mL LPS in the presence or absence of azilsartan (5, 10 μM) for 24 h.

U937 cells were pretreated with SeMet (1 μM) and subsequently induced with LPS (1 μg/ml) for 24 h. In the cell counting kit-8 assay (CCK-8), SeMet significantly inhibits the proliferation of U937 cells. SeMet also inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) stimulated by LPS.

In U937 cells, AST has been shown to attenuate intracellular O2− production by restoring the antioxidant function of superoxide dismutase and catalase, consequently reversing ROS production and...

In LPS-sensitive U937 cells, LPS from E. coli is an agonist of TLR4. In this study, this led to a dose-dependent induction of the abovementioned inflammatory marker genes in the U937 macrophages.

2020年1月15日 · In this study, we found that omentin-1 inhibited LPS-induced expression of inflammatory mediators and pro-inflammatory cytokines in macrophages. Furthermore, omentin-1 inhibited activation of the NF-κB pathway by suppressing both nuclear p65 accumulation and transfected NFκB promoter activity.

2021年3月24日 · Our results indicate that LPS-induced pro-inflammatory responses are enhanced by bead phagocytosis regardless of the uptake mechanism. Additionally, phagocytosis enhanced LPS-induced NF-κB...

2011年9月27日 · Monocytic cells (U-937) were cultured in the presence of 30 mmol/L glucose and stimulated with 0.5 ng/μL LPS in the presence or absence of C-peptide (1 μmol/L) for 24 h to induce inflammatory cytokine secretion. Adhesion of U-937 monocytes to human aortic endothelial cells (HAEC) was also studied in the presence or absence of C-peptide.

Results: Addition of C-peptide significantly reduced cytokine secretion from LPS-stimulated U-937 monocytes. Adhesion of U-937 cells to HAEC was also significantly reduced by C-peptide. These effects were accompanied by reduced NF-κB p65/p50 nuclear translocation and decreased phosphorylation of IκB-α.

We report that COX-2 expression is up-regulated in phorbol ester (phorbol myristate acetate, PMA)-differentiated human U937 macrophage-like cells stimulated with lipopolysaccharide (LPS), whereas COX-1 is not up-regulated.

1994年6月23日 · LPS-mediated PLD activation of THP-1 or U-937 was inhibited by staurosporine (2 μM) and by protein kinase C (PKC) down-regulation with 12- O -tetradecanoylphorbol 13-acetate (TPA) suggesting a role for PKC.