2025年2月4日 · LFA1 clusters co-trafficked with LC3, facilitating LFA1 accumulation at the contact surface. LC3b knockout decreased lymphocyte adhesiveness. LFA1 activation did not induce autophagy, whereas it...

2024年2月7日 · We assayed LC3B conjugation activity in a small unilamellar vesicle (SUV) system similar to that originally used to demonstrate Atg8 conjugation activity of the Atg12–Atg5-Atg16-Atg3 complex . Here, purified human proteins were used (1.0 μM), WIPI2 (0.5 μM) was included in the protein mixture, and 10% PI(3)P was included in the SUVs ( 18 ).

2021年8月9日 · While our studies show that phosphorylation of LC3B was critical for driving the proper retrograde transport in LC3B T50A particles, T50E-positive particles revealed an unusually high frequency of directional switches in primary neurons and in HeLa LC3B-KO cells.

2024年8月27日 · Cell-based and in vitro reconstitution experiments show that S1R interacts with the 3′ UTR of LC3 mRNA and ribosomes to promote LC3 translation. Strikingly, the 3′ UTR of LC3 is also required for LC3 protein lipidation, thereby linking the mRNA-3′ UTR to LC3 function.

2024年6月4日 · Here we combined two complementary reconstitution approaches to reveal the function of LC3B and its ligase complex during phagophore expansion. We found that LC3B forms together with...

2019年2月18日 · Here we have purified the full-length human ATG12-5–ATG16L1 complex and show its essential role in LC3B/GABARAP lipidation in vitro. We have identified two functionally distinct membrane-binding...

在哺乳动物中，存在 4 种lc3异构体（lc3a、 lc3b 、 lc3b2和 lc3c ），在不同的组织细胞中表达会有差异。目前主要研究的是lc3a和lc3b，lc3c在许多细胞中表达量很低，研究的也较少。

2025年2月1日 · LC3B drives autophagy via interaction with autophagy-related proteins, autophagosome-lysosome fusion, and transcriptional cofactor activity. LC3B modulates apoptosis, acting as a context-dependent regulator. Elevated LC3B levels in solid tumors highlight its potential as a cancer biomarker.

lc3b是研究最多的一个亚型，也是公认的自噬关键指标。lc3b的前体形式为prolc3b，被 atg4蛋白 裂解后生成lc3b-i（约16kda），lc3b-i被 atg7 和 atg3 修饰和加工后，再与 磷脂酰乙醇胺 (pe)结合，生成lc3b-ii（约14kda）， lc3b-ii是自噬体标记的关键蛋白之一。

2021年1月1日 · We identified USP10 in a CRISPR-Cas9 knockout screen for ubiquitination-related genes that regulate LC3B levels. Biochemical analyses showed that silencing of USP10 reduces the levels of both the LC3B-I and LC3B-II forms of LC3B through increased ubiquitination and proteasomal degradation.