关于『细胞自噬』，看这一篇就够了！ - 知乎专栏
在自噬体形成过程中，p62作为链接LC3和聚泛素化蛋白之间的桥梁，被选择性地包裹进自噬体，之后被自噬溶酶体中的蛋白水解酶将其降解（图9），所以p62蛋白的表达量与自噬活性呈 …
自噬的检测方法（二） - 知乎 - 知乎专栏
研究表明，p62蛋白锌指结构域和UBA结构域之间的连接区域（LRS区域）负责与自噬受体蛋白 Atg8/LC3结合，UBA结构域负责招募泛素化蛋白。 在自噬体形成过程中，p62作为链接LC3和 …
搞定这 2 个明星分子 LC3、p62，看清自噬真面目（附信号通路图）
Sep 21, 2023 · p62 是研究广泛的一个自噬底物。在自噬体形成过程中，p62 作为连接 LC3 和聚泛素化蛋白之间的桥梁，被选择性地包裹进自噬体，之后被自噬溶酶体中的蛋白水解酶降解（图 …
Tracking Autophagy With LC3B & p62 - Thermo Fisher Scientific
The p62 protein is able to bind ubiquitin and also to LC3, thereby targeting the autophagosome and facilitating clearance of ubiquitinated proteins. Invitrogen Molecular Probes tools enable: …
LC3- and p62-based biochemical methods for the analysis of ... - PubMed
Immunoblot analysis of LC3 and p62/SQSTM1, among other proteins, has been widely used to monitor autophagic flux. Here, we describe basic protocols to measure the levels of …
S -acylation of p62 promotes p62 droplet recruitment into ... - Cell …
Oct 5, 2023 · We propose that S-acylation enhances autophagic membrane association of p62 droplets and hence promotes wetting of p62 droplets with the autophagic membrane, thereby …
使用LC3B和p62来追踪自噬过程 - 赛默飞世尔科技公司
我们提供了可进行LC3B及p62定位的特异性检测工具，用于自噬诱导、蛋白聚合物清除、细胞自噬过程研究。 而LC3B标记则可追踪p62的结合及随后的自噬体补充过程。
LC3- and p62-based biochemical methods for the analysis of …
Mar 15, 2015 · Here, we outline detailed methodologies for LC3- and p62-based biochemical analysis of autophagy progression. We also discuss possible pitfalls and important cautions …
mRNA and protein dataset of autophagy markers (LC3 and p62…
Mar 9, 2016 · Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by …
p62/SQSTM1 binds directly to Atg8/LC3 to facilitate ... - PubMed
Aug 17, 2007 · The specific interaction between p62 and LC3, requiring the motif we have mapped, is instrumental in mediating autophagic degradation of the p62-positive bodies. We …