2025年2月4日 · Inhibiting Mst1 kinase-mediated LC3 phosphorylation promoted LC3-mediated LFA1 recruitment to the contact surface through direct interaction with RAPL, uncovering an unprecedented integrin...

2021年4月18日， 中山大学附属第三医院 消化内科吴斌教授研究团队在自噬领域专业期刊 《Autophagy》（2020年影响因子16.016） 在线发表了题为“HBx induces hepatocellular carcinogenesis through ARRB1-mediated autophagy to drive the G1/S cycle”的研究论文。 该研究阐明了乙肝相关性肝癌癌变过程中的新机制，为乙肝相关性肝癌的防治提供了重要的理论依据及潜在的治疗靶点。 ·LC3荧光双标腺病毒系统（Ad-mCherry-GFP-LC3，GCD0165968):

We examined the effects of GFP-LC3 expression on autophagy, acinar cell function, and experimental pancreatitis. Unexpectedly, GFP-LC3 expression markedly increased endogenous LC3-II level in pancreas, caused by downregulation of ATG4B, the protease that deconjugates/delipidates LC3-II.

LC3 is a marker protein that is involved in the formation of autophagosomes and autolysosomes, which are usually characterized and monitored by fluorescence microscopy using fluorescent protein-tagged LC3 probes (FP-LC3). FP-LC3 and even endogenous LC3 can also be incorporated into intracellular protein aggregates in an autophagy-independent ...

6 天之前 · lc3ii免疫荧光是可行的，也有一些文献在做。相比于gfp-lc3稳定株或者病毒感染（当然瞬转的不评价）有一定鉴定优势，但是不能体现一种自噬流聚集的直观过程感受，不知道你能不能理解。

2021年8月21日 · Immunostaining for Gfap on retinal cryosections of Tg(CMV:GFP-Lc3) autophagy reporter fish, three days after ONC. Representative images disclosed a clear co-localization of the Lc3 (green) and Gfap (red) signal in the IPL, as well as in the NFL, where the end feet of the Müller glia are positioned.

2008年6月1日 · LC3 is the mammalian autophagic protein that localizes to the autophagosome membrane as well as to the cytosol (Kabeya et al., 2000). Overexpression of GFP tagged LC3 (GFP-LC3) is a well-accepted, straightforward and specific assessment of autophagosome formation without perturbing autophagosome number or function (12).

2022年3月17日 · Microtubule-associated protein 1 light chain 3 (LC3), a ubiquitin-like protein that is important for the formation of the autophagosome, is a reliable marker to monitor autophagy. The behavior of GFP-LC3 fusion protein is very similar to the endogenous LC3, which has been widely used as a marker to monitor autophagy [7, 8]. An increased number ...

利多卡因增加了 cci 大鼠中 lc3/gfap 阳性细胞的数量。 此外，利多卡因抑制 GFAP 和 p62 的表达，并增强 LPS 处理的星形胶质细胞中 LC3-II/LC3-I 的表达。 利多卡因抑制 LPS 处理的星形胶质细胞中 TNF-α 和 IL-1β 的水平。

In this chapter, we will detail step-by-step the GFP-LC3 reporter protocol we have adapted in our lab. This protocol begins with the generation of lentivirus expressing GFP-LC3. Then, the cells are transduced with titrated virus.