2025年3月30日 · This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter ...

2023年4月5日 · The GLS1 gene gives rise to two splicing variants, kidney glutaminase (KGA) and glutaminase C (GAC), with a shared N-terminal sequence (1–550) but distinct C-terminal sections. The amino acid sequence from Ile221 to Leu533 has been identified as the catalytic domain of GLS1, as illustrated in Fig. 1 A.

2023年10月13日 · Glutaminase catalyzes the first step of glutamine metabolism, converting glutamine to glutamate, which enters the tricarboxylic acid cycle. In human, glutaminase is encoded by two different genes,...

Our data demonstrate increased expression of GLS1 isoforms KGA and GAC with mitochondrial localization in latently EBV-infected cells and de novo EBV-infected PBMCs. c-Myc upregulates KGA and GAC protein levels, which in turn elevate the levels of intracellular glutamate.

2024年3月4日 · Gls encodes kidney-type glutaminase (KGA) and its C-terminal truncated splice variant glutaminase C (GAC) which is highly expressed in many cancers. Gls 2 encodes only one active isozyme,...

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter ...

2023年10月16日 · GLS1可翻译产生两种不同的活性形式：肾谷氨酰胺酶A（KGA）和高度活化的谷氨酰胺酶亚型C（GAC），其中GAC亚型在多种癌症中因其对谷氨酰胺的高度利用而高度表达。 已有研究表明谷氨酰胺酶的抑制显示出抑制癌症细胞生长和诱导细胞凋亡的潜力，因此成为癌症治疗中备受瞩目的治疗靶点。 目前，一种特定的谷氨酰胺酶抑制剂正在进行多项临床试验。...

2014年1月23日 · Here we report the active site inhibition mechanism of KGA through the crystal structure of the catalytic domain of KGA (cKGA) in complex with 6-diazo-5-oxo-L-norleucine (DON), a substrate...

作为催化谷氨酰胺分解的第一种酶，人肾型谷氨酰胺酶异构体 (KGA) 正成为 BPTES [bis-2- (5 phenylacetamido-1, 2, 4-thiadiazol-2-yl)ethyl硫化物]，尽管 KGA 的调控机制尚不清楚。 基于晶体结构，我们揭示了 BPTES 与 KGA 二聚体界面处的变构口袋结合，触发了催化位点附近关键环（Glu312-Pro329）的显着构象变化并使其失活。 BPTES 在疏水口袋上的结合模式解释了它对 KGA 的特异性。 有趣的是，细胞中的 KGA 活性受 EGF 刺激，和 KGA 与 Raf-1/Mek2/Erk 信 …

Increased expression of rat kidney-type glutaminase (KGA) during metabolic acidosis results from selective mRNA stabilization. This process is mediated by an 8-base AU-sequence that functions as a pH-response element (pHRE).