
pJet1.2 kanR cassette - Addgene
Plasmid pJet1.2 kanR cassette from Dr. Stephan Gruber's lab contains the insert Kanamycin resistance cassette for Bacillus subtilis selection and is published in Methods Mol Biol. 2019;2004:49-61. doi: 10.1007/978-1-4939-9520-2_5.
【质粒介绍】分解篇2-抗生素 - 知乎
这一篇我们要为大家讲解的就是 抗性基因 (resistant gene) 在质粒图谱中的AmpR、KanR等表示 质粒载体 中的筛选标签,多为抗生素抗性基因,方便后续通过抗生素筛选阳性克隆。 只存在单抗性的质粒多为 原核克隆载体 和 原核表达载体。
Here is a part named NeoR – KanR on MolecularCloud
2020年10月21日 · Part Name: NeoR - KanR Description: Aminoglycoside phosphotransferase from Tn5 confers resistance to kanamycin in bacteria, or neomycin and G418 (Geneticin (R)) in eukarytoes.
Sequence analysis and plasmid mobilization of a 6.6-kb kanamycin ...
2022年7月22日 · To further understand the diversity of these KanR plasmids, we present here the sequence analysis of the pSNC3-Kan plasmid from S. Newport strain ARS #574, and evaluation of the mobilization of four representative KanR plasmids by F and IncP conjugal transfer systems.
The effects of kanamycin concentration on gene transcription …
Kanamycin, an aminoglycoside antibiotic, can inhibit the peptide synthesis of E. coli by blocking the translocation process (Semenkov et al. 1982). In this work, green fluorescent protein was selected as a reporter, and kanamycin was selected as a representative for antibiotics.
pUC57-Kan Sequence and Map - SnapGene
Cloning vector with a kanamycin resistance marker, suitable for generating ExoIII deletions. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from …
Development of CRISPR-Cas13a-based antimicrobials capable of ... - Nature
2020年6月10日 · Finally, the kanamycin (Km) resistance gene (KanR) was inserted as a selection marker to generate the constructs of PICI-based EC-CapsidCas13a::KanR_ blaIMP-1 and EC-CapsidCas13a::KanR ...
Exceptional segregation of a selectable marker (KanR) in …
Genetic analysis of the kanamycin-resistance (KanR) trait in >900 independent transformants of Arabidopsis revealed that 9% produced progeny families with an enormous deficiency of KanR individuals.
Engineering of a functional thermostable kanamycin resistance …
A transformation system for Moorella thermoacetica ATCC39073 was developed using thermostable kanamycin resistant gene (kanR) derived from the plasmid pJH1 that Streptococcus faecalis harbored.
The resDNASEQ system enables rapid, sensitive, and specifi c quantitation of plasmid DNA containing the kanamycin resistance gene (KanR) by targeting multiple KanR variants present on commonly used plasmids.