Expansion of NK Cells Using Genetically Engineered K562 Feeder …
Here, we describe an in vitro expansion method of human NK cells among PBMCs by coculturing with GE_K562 cells. Keywords: Expansion; Feeder cells; K562; Natural killer cell. Natural killer …
自然杀伤（NK）细胞的来源、扩增技术以及符合GMP标准的制造 …
缺乏人类白细胞抗原表达的K562细胞通过激活共刺激信号促进NK细胞的增殖。 据报道，当K562细胞作为饲养细胞与IL-2和IL-15联合使用时，NK细胞的扩增超过300倍。 此外，肿瘤细胞膜颗 …
使用基因工程K562饲养细胞扩增NK细胞。,Methods in Molecular …
2016年5月15日 · 博士 达里奥·坎帕纳（Dario Campana）的小组发现，由K562细胞共表达的膜结合IL-15和4-1BBL协同作用，增强了K562特异性NK的刺激能力，导致外周 …
Frontiers | Expansion of Human NK Cells Using K562 Cells …
2019年4月23日 · Here, we report a new effective method to expand human NK cells ex vivo using K562 cells genetically engineered (GE) to express OX40 ligand (K562-OX40L) in combination …
Allogeneic NK cells with a bispecific innate cell engager in
3 天之前 · NK cells were isolated from the CB units, preactivated with IL-12 (10 ng ml −1), IL-15 (50 ng ml −1) and IL-18 (50 ng ml −1) for 16 h and expanded using K562-based feeder cells …
An Extended Flow Cytometry Evaluation of ex Vivo Expanded NK …
2024年11月1日 · Successful transduction of mbIL-21 and 4-1BBL supports growth and survival of natural killer (NK) cells during expansion. Feeder cells create an optimized microenvironment, …
Expansion of Human NK Cells Using K562 Cells Expressing OX40 …
2019年4月24日 · Here, we report a new effective method to expand human NK cells ex vivo using K562 cells genetically engineered (GE) to express OX40 ligand (K562-OX40L) in combination …
《K562工程细胞系的构建及对NK-92细胞体外扩增的研究》 - 豆丁网
k562工程细胞系与nk-92细胞的共培养体系在临床治疗上具有显著的优势。首先，这一体系可以实现对免疫细胞的精确调控，从而提高其抗肿瘤、抗感染等能力。其次，这一体系还可以用于药 …
Ex Vivo Expansion of Human NK Cells Using K562 Engineered to
2016年5月14日 · Data shows the cytotoxic potential of expanded human NK cells against—K562 (chronic myeloid leukemia cell line), A375 (malignant melanoma), IMR32 and CHP134 …
Cell-Membrane-Anchored DNA Nanoplatform for Programming
4 天之前 · Quantify the assembly of NK cell–K562 cell using flow cytometry. 7. Calculate the assembly efficiency of NK cell–K562 cell with the ratio of amounts of assembled NK cells to …