CD3 receptor modulation in Jurkat leukemic cell line - PubMed
To confirm these findings, PKC activity was estimated in Jurkat clones. Our data demonstrated that Jurkat clones with different CD3 expression showed also different PKC activities, so we conclude that PKC-dependent pathway is the main way of controlling CD3 level on Jurkat clones.
Flow Cytometry: CD3 Market in Jurkat T Cells? - ResearchGate
I am looking for a protocol for the stimulation of Jurkat T cells with soluble anti-CD3 and ati-CD28 antibodies.
T cell activation and effector function in the human Jurkat T cell ...
In vitro easy-to-handle cellular models are valuable tools to study intracellular molecular mechanisms in live cells. The CD4 T cell line Jurkat (JK) has been widely employed to investigate intracellular signaling leading to T cell activation in response to T cell receptor (TCR) triggering.
Molecular pathway profiling of T lymphocyte signal transduction ...
Jurkat T cells were stimulated with PMA, CD28 and CD3 alone and combinations thereof in the presence of the above mentioned inhibitors. Based on the results of the first gene expression profiling experiment, we chose evaluated gene expression after 8 hours of stimulation.
Harnessing CD3 diversity to optimize CAR T cells - Nature
2023年11月6日 · In the present study, we have systematically engineered new CD3 CARs, each containing only one of the CD3 intracellular domains. We found that CARs containing CD3δ, CD3ε or CD3γ...
Activated T cell-derived exosomal PD-1 attenuates PD-L1 ... - Nature
2021年6月25日 · To measuring the alteration of activation level in T cells, we generated NFAT-Luciferase-transfected Jurkat-PD-1 cells and treated with anti-CD3/CD28/IgG (stimulatory) or...
CRISPR/Cas9-medaited knockout of endogenous T-cell receptor in Jurkat …
2022年9月1日 · In this study, we use CRISPR/Cas9 to knock out both the α and β chains of the endogenous TCRs of Jurkat cells.
CD3 receptor modulation in Jurkat leukemic cell line
2004年2月1日 · In our study we examined the role of two main T cell signal transduction pathways in controlling the surface level of CD3 antigen, one based on protein kinase C activity and the other dependent...
Jurkat T cells and development of the T-cell receptor ... - Nature
2004年4月1日 · Although the nature of the association between the αβ-TCR and CD3 polypeptides remained mysterious, the observation that CD3-specific antibodies were stimulatory for human T cells prompted ...
Bivalent binding of an anti-CD3 antibody to Jurkat cells induces ...
1989年8月1日 · Ligand binding and cross-linking of TCR/CD3 complex leads to T cell stimulation in immunologic responses. As a prelude to investigating the dynamic interactions of these receptors, we have characterized binding of the mAb OKT3 specific for CD3 on Jurkat cells.