In order to be able to examine the co-distribution of two (or more) different antigens in the same sample, a double immunofluorescence procedure can be carried out. Primary antibodies …

Double staining of membrane and nuclear antigens using consecutive immunoreactions. ( a ) Depicts a large B-cell lymphoma (LBCL) doubly stained for the B-cell-associated membrane …

Jan 23, 2024 · If both antibodies survive the “double pretreatments”, you are ready for immunohistochemistry double staining. Another alternative is to do pretreatments separately …

Here, we describe two common techniques for immunofluorescent (IF) staining using multiple antibodies in the same assay. Below is a general protocol overview f or indirect IF using …

Jul 3, 2021 · This video lecture describes How to perform co-immunofluorescence staining or double immunofluorescence staining using the two antibodies from same source sp...

We provide a protocol for immunofluorescent double staining incubating the antibodies together. Cell lines, cytology smears, cytospin preparations. Coat coverslips with polyethylineimine or …

I would like to do a double immunofluorescence staining TOM20 and LC3 but both primary antibodies is produced in rabbit. Can anyone help me with a protocol?

In this post, we present several techniques for double-IF staining using various combinations of primary antibodies, as well as some general guidelines for optimizing and troubleshooting …

Double staining for Immunofluorescence Microscopy In order to be able to examine the co-distribution of two (or more) different antigens in the same sample, a double …

The double-immunofluorescence or triple-immunofluorescence technique uses two or three primary antibodies raised from hosts of different species. Primary antibodies are mixed at pre …