2022年4月6日 · Here, we undertook an integrative multiomics approach to delineate the process of iMPC reprogramming in comparison to myogenic transdifferentiation mediated solely by MyoD. Using transcriptomics, proteomics, and genome-wide chromatin accessibility assays, we unravel distinct molecular trajectories that govern the two processes.

2022年8月16日 · Focusing on the three-dimensional (3D) chromatin interactions, we show that MyoD directly establishes chromatin loops to activate myogenic transcriptional program. Similarly, dynamic changes of CTCF-mediated chromatin interactions are favorable for fibroblast-to-myoblast conversion.

2021年1月22日 · In this study, we hypothesized that MyoD expression in proliferative myoblasts-induced chromatin changes that enhanced MyoD function on chromatin. As an initial approach to confirm this hypothesis, we performed a comprehensive genome-wide-analysis to reveal the unique chromatin state around MyoD binding sites to determine the chromatin ...

2001年10月12日 · We used expression arrays and chromatin immunoprecipitation assays to demonstrate that myogenesis consists of discrete subprograms of gene expression regulated by MyoD. Approximately 5% of assayed genes alter expression in a specific temporal sequence, and more than 1% are regulated by MyoD without the synthesis of additional transcription factors.

2022年11月9日 · TCF12 contributes to muscle development and regeneration and works with MYOD to govern myogenic genes at gene regulatory regions through chromatin remodelling. Muscle development and regeneration are sophisticated processes.

Validation between DNA methylation and gene expression in the MyoD promoter and distal enhancer region. A) 22 methylation sites including a part of the promoter and first exon region (217 ; 2233...

In this study, we demonstrate that protein engineering of the master transcription factor MyoD can enhance the conversion of human dermal fibroblasts and adult stem cells to a skeletal myocyte phenotype.

若要评估MyoD和IGF信号通路之间的潜在相互作用，我们研究了通过定量感染编码小鼠MyoD的重组腺病毒急性转化为成肌细胞的C3H 10T1 / 2成纤维细胞的肌肉分化。

2010年4月20日 · MyoD is a classic master regulator that commits those somite cells in which it is expressed to a myoblast fate and also later activates skeletal muscle genes during the differentiation of these cells.

2022年4月6日 · Transient MyoD overexpression in concert with small molecule treatment reprograms mouse fibroblasts into induced myogenic progenitor cells (iMPCs). However, the molecular landscape and mechanisms orchestrating this cellular conversion remain unknown.