A typical ITC experiment contains three steps: (i) a ligand is titrated into a solution containing the bio-macromolecule (e.g., protein) of interest; (ii) the heat released or absorbed that is …

2018年10月10日 · In the following, this mini-review will introduce how to apply ITC measurement to reveal the thermodynamic and kinetic signature of ligand–target binding, providing important …

2023年3月9日 · Isothermal titration calorimetry (ITC) has become the gold standard for studying molecular interactions in solution. Although it is increasingly being used in the soft...

In thermodynamical analysis, Isothermal Titration Calorimetry (ITC) is considered as a golden standard because it measures unlabeled partner interactions with high precision, but also …

2018年3月1日 · Here we present a pair of rapid, complementary ITC methods that simultaneously measure inhibitor association and dissociation rates and the inhibitory constant Ki, for enzyme …

2016年5月1日 · ITC is a powerful technique that can reliably assess the thermodynamic underpinnings of a wide range of binding events. When metal ions are involved, complications …

Figure 1: ITC allows the rapid and accurate assessment of binding reactions, without the need to develop new assay protocols for each biomolecule or ligand. The stoichiometry of binding, and …

2020年11月3日 · Isothermal titration calorimetry (ITC) is a powerful tool for determining the affinity and the thermodynamic parameters of ligand binding from a single...

By accessing the enthalpy change, we get a step closer in estimating the driving forces that characterize the interaction of a protein with a ligand, information much needed in the drug …

Riboswitches regulate genes by binding to small-molecule effectors. Isothermal titration calorimetry (ITC) provides a label-free method to quantify the equilibrium association constant, …