
SCI作图进阶版GraphPad绘图技能2.0:IC50的log转化技巧 - 知乎
设置X轴格式,因为X轴中的数字是经过log10计算的,因此在Regularly spaced ticks-Number format里选择 Antilog,即可获得X轴的浓度梯度值。 到这里,一个浓度梯度等距排列的折线图就完成啦。 当然,我们还可以继续设置次刻度标记,来丰富X轴中的内容。 注意,次刻度标记也要勾选log哦! 整个操作还是非常简单便捷的。 为了使大家更好的理解,我们以个性化的数据为例:首先将X轴(浓度)的数据进行log10转换并输入到对应的表格中,Y轴不变。 然后对横坐标进 …
如何用GraphPad Prism计算IC50值及绘制量效关系曲线 - 知乎
半抑制浓度 (或称半抑制率),即 IC50 ,英文名称为half maximal inhibitory concentration。它能指示某一药物或者物质(抑制剂)在抑制某些生物程序(或者是包含在此程序中的某些物质,比如酶、细胞受体或是微生物)的半量。
IC50 - Wikipedia
Half maximal inhibitory concentration (IC50) is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. IC 50 is a quantitative measure that indicates how much of a particular inhibitory substance (e.g. drug) is needed to inhibit, in vitro, a given biological process or biological component by 50%. [1] .
半数抑制浓度 - 维基百科,自由的百科全书
推导IC 50 值:纵轴排列数据抑制,横轴排列log(浓度);然后确定最大和最小抑制;IC 50 就是曲线通过50%抑制水平时的浓度。 半数抑制浓度 ( IC 50 )是物质抑制特定生物或生化功能的 效力 的量度。
GraphPad Prism软件计算IC50与作图 - CSDN博客
2022年5月12日 · 在生物与医药领域,某种先导化合物的IC50值非常重要,而通过GraphPad Prism软件计算IC50值,是一种十分简便的方法,其导出的数据图,可以直接用来发文章。
【求助】graphpad prism5 计算IC50曲线时什么时候用normalized …
2014年2月23日 · 请教一下,使用prism软件计算IC50的时候,什么时候该用log(inhibitor) vs.normalized response-variable slope,什么时候该用log(inhibit
IC50的计算常用的2种方法 - 知乎 - 知乎专栏
IC50(half maximal inhibitory concentration),即半抑制浓度,指某一种物质对某些生物程序抑制达到50%抑制效果时的浓度。 对细胞增殖方面,可以理解为对细胞增殖的抑制效果达到细胞正常增殖水平50%时的药物浓度。
Equation: log (inhibitor) vs. response - GraphPad
Many log (inhibitor) vs. response curves follow the familiar symmetrical sigmoidal shape. The goal is to determine the IC50 of the inhibitor - the concentration that provokes a response half way between the maximal (Top) response and the maximally inhibited (Bottom) response.
IC50 Calculator - AAT Bioquest
This online calculator is used to determine and graph the IC50 (half maximal inhibitory concentration) value given a set of experimental data. Data can be from Excel or CSV. Calculator gives equation of four-parameter logistic (4PL) curve as well as graph.
GraphPad Prism 10 Curve Fitting Guide - Equation: Absolute IC50
The EC50 (or IC50) refer to a concentration of agonist (or antagonist) required to increase (or reduce) the measured response to half - or 50% - of its maximal value. However, there are a number of ways that this concept of "half" can be interpreted.