2014年4月17日 · In Huh7 hepatic cells, treatment with PA for 8 h triggered activation of both unfolding protein response and the autophagic flux. Conversely, prolonged treatment with PA (24 h) induced ER stress...

Download scientific diagram | Co-localization of GFP-LC3 and LAMP1 proteins in Huh7 cells with and without DV2 infection or with rapamycin treatment. Huh7 cells were transfected with...

2018年9月5日 · Alcohol treatment could induce autophagy in Huh7 cells (a hepatoma cell line that supports HCV JFH-1 replication), evidenced by the increase of LC3B-II levels, the conversion of LC3B-I to...

2018年6月6日 · As Rab7-PLEKHM1-LC3 complex plays a critical role in autophagosome-lysosome fusion, we assume that Rab7-PLEKHM1-LC3 complex can regulate HBV autophagic degradation to some extent (Fig. 2 H). Finally, the effects of Rab7 silencing on HBV transcription and promoter activity were determined.

2024年12月3日 · In Huh7 ECD-GFP cells, the coincidence area of LC3 (red) and lysosomes (green) was greater than that in the control group, as analyzed using the plot profile tool in ImageJ (Fig. 4F). To observe the autophagosome more accurately, we transferred the LC3 double fluorescent plasmid pCMV-mCherry-GFP-LC3B into Huh7 Ctrl GFP and Huh7 ECD-GFP cells.

The results showed that the number of LC3-positive autophagosomes increased significantly in Huh7 cells after infection by ZIKV. The expression of LC3-Ⅱ increased significantly from 12 h post...

2021年10月1日 · Expression of LC3B, CYP2E1, and NOX4 was studied in a mouse model of acute ethanol exposure by immunoblotting and immunohistochemistry. Autophagy was further studied in primary mouse hepatocytes and huh7 cells in response to ethanol and its major intermediator acetaldehyde.

We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation...

Huh7细胞用5~40 μmol/L姜黄素培养48 h，通过蛋白质印迹法检测自噬微管相关蛋白1轻链3（LC3）-Ⅱ/LC3-Ⅰ的比值，免疫荧光法检测自噬小体的形成，评估Huh7细胞自噬水平的变化。

Huh7细胞用5～40μmol/L姜黄素培养48 h,通过蛋白质印迹法检测自噬微管相关蛋白1轻链3 (LC3)-Ⅱ/LC3-Ⅰ的比值,免疫荧光法检测自噬小体的形成,评估Huh7细胞自噬水平的变化。 通过流式细胞术检测Huh7细胞凋亡情况。 加入5 mmol/L自噬抑制剂3-甲基嘌呤 (3-MA)和20μmol/L姜黄素培养Huh7细胞,检测Huh7细胞凋亡水平和自噬水平的变化。 结果 CCK-8检测显示姜黄素能抑制Huh7细胞的增殖 (P<0.05,P<0.01),且细胞凋亡率随着姜黄素浓度的升高而上升;蛋白质印迹检测显示加 …