2020年1月8日 · Functional mouse hematopoietic stem cells (HSCs) are expanded 236- to 899-fold ex vivo using a fully defined albumin-free culture system.

2016年8月25日 · Here, we present 1656 single HSPC transcriptomes analyzed by single-cell RNA sequencing (scRNA-seq) with broad gates, deep sequencing, and index sorting to retrospectively identify populations by surface marker expression.

The Lineage Cell Depletion Kit, mouse, and the Direct Lineage Cell Depletion Kit, mouse, enable fast preenrichment of mouse HSCs from bone marrow by depleting cells expressing lineage markers. Designed to magnetically label mature cells (T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythroid cells) and their ...

Challenges of the humanized mouse model include finding suitable sources for human hematopoietic stem cells (HSC) and reaching sufficient engraftment of these cells in immunocompromised mice. Methods: In this study, we compared the use of CD34 + HSC from cord blood (CB) vs HSC from adult mobilized peripheral blood.

Hematopoietic stem cells (HSCs) remain by far the most well-characterized adult stem cell population both in terms of markers for purification and assays to assess functional potential. However, despite over 40 years of research, working with HSCs in the mouse remains difficult because of the relati …

2008年11月20日 · In this review, we will discuss a range of methods for characterizing HSCs, progenitors, and mature hematopoietic cells, which can then be applied to the analysis of mutant mice or nonsteady state conditions.

2019年5月29日 · Here we describe the development of a defined, albumin-free culture system that supports the long-term ex vivo expansion of functional mouse HSCs. We used a systematic optimization approach, and...

2024年2月27日 · In vivo hematopoietic stem cell (HSC) gene therapy is an emerging and promising area of focus in the gene therapy field. Humanized mouse models are frequently used to evaluate novel HSC gene therapy approaches. Here, we comprehensively evaluated 2 mouse strains, NSG and NBSGW.

2012年2月13日 · Humanized mouse models that have received human cells or tissue transplants are extremely useful in basic and applied human disease research. Highly immunodeficient mice, which do not reject...

Here we present a new microphysiological system (MPS) to investigate how hypoxia affects hISCs isolated from healthy human tissues. We then test the hypothesis that some inflammation-associated interleukins protect hISCs during prolonged hypoxia. Methods: hISCs were exposed to <1.0% oxygen in the MPS for 6-, 24-, 48- & 72hrs.