5' Hexynyl Return to Attachment Chemistry / Linkers Modifications. This modification is the simplest way to introduce a 5' terminal alkyne group. Alkynes readily react with azides in the …

The 5'-Hexynyl modifier can be used to incorporate an active alkyne onto the 5'-end of an oligonucleotide. The alkyne is separated from the 5'-end nucleotide base by a 4-carbon spacer …

2011年9月12日 · After synthesis, deprotection, and initial purification, the alkyne group is reacted with a modification containing an azide functional group. All 5' click modifications conjugate …

Oligonucleotides containing the 5’-Hexynyl moiety are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. A number of azide labels are …

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