Explore how to analyze proteins with HaloTag® Technology. Start with a single protein fusion with HaloTag® protein and you can purify proteins, investigate protein:protein and protein:DNA interactions, image cells and more.

2015年5月14日 · In this review, we examine all current applications of the HaloTag technology platform for biomedical applications, such as the study of protein isolation and purification, protein function, protein–protein and protein–DNA interactions, biological assays, in vitro cellular imaging, and in vivo molecular imaging.

2008年6月6日 · The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (HaloTag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces.

HaloTag® Fluorescent Ligands offer fluorescent labeling, affinity tagging and solid-phase attachment abilities. The ligands form covalent bonds rapidly under general physiological conditions; the bonds are highly specific and essentially irreversible.

2021年12月16日 · The HaloTag variants developed enable live-cell fluorescence lifetime multiplexing of three species in a single spectral channel, freeing up other spectral channels for further multiplexing.

As an alternative approach, the innovative HaloTag protein fusion platform allows protein function and interaction to be comprehensively analyzed using a single genetic construct with multiple capabilities.

2015年4月9日 · In this review, we examine all current applications of the HaloTag technology platform for biomedical applications, such as the study of protein isolation and puri fication, protein function, protein protein and protein DNA − − interactions, biological assays, in vitro cellular imaging, and in vivo molecular imaging.

HaloTag®技术为在活细胞中和体外进行快速、定点标记HaloTag®融合蛋白提供了新的选择。 该技术基于HaloTag®蛋白和合成配基之间形成的共价键，这些配基具有多种功能，包括荧光标记、亲和标记和附着在固相载体上；而共价键在生理条件下可迅速形成，且具有高度特异性和本质上的不可逆，形成的复合物即使在变性条件下也是稳定的。 因此，使用HaloTag®只需要一个可以表 …

This modular technology is based on the formation of a covalent bond between a fusion tag, HaloTag® reporter protein, and synthetic ligands, and enables complete characterization of protein function in cellular and biochemical environments.

The HaloTag is a 34 KDa monomeric protein derived from a bacterial haloalkane dehalogenase. The majority of fusion tags in use today utilize a reversible binding interaction with a specific ligand. The HaloTag system is unique in that it forms a covalent linkage to its chloroalkane ligand.