The HA-tag is a protein tag derived from the human influenza hemagglutinin (HA) protein, which allows the virus to target and enter host cells. An HA-tag is composed of a peptide derived from the HA-molecule corresponding to amino acids 98-106, which can be recognized and selectively bound by commercially available antibodies .

The HA-tag allows simple and efficient affinity purification of the tagged protein using HA-tag specific antibody conjugated to agarose-beads. The HA-tag (YPYDVPDYA-tag) also can be used for detection in western blot by using a HA-tag-specific antibody.

Commonly used peptide tags are HA (derived from the hemagglutinin protein of influenza virus), Myc, V5, and DYKDDDDK. Even though they are most routinely used for detection, some peptide tags, such as DYKDDDDK, are also used for purification of the fused protein.

Explore widely-used epitope tags, such as c-myc and HA, including recommended applications and what to watch out for.

Since the HA-tag sequence corresponds to the 98-106 residues of the immunogenic human influenza hemagglutinin protein, this protein is a spike membrane glycoprotein and can be recognized by a variety of antibodies such as HA-7, C29F4, and 16B12.

2007年2月1日 · From a more positive point of view, our observations open the way to the use of the HA tag as a bona fide caspase 3/7 sensor responding by immunoreactivity loss with a good signal-to-noise...

2025年3月13日 · Explore the structural characteristics of HA-tag, its role in antibody binding, and commonly used laboratory techniques for its detection. Researchers use epitope tags to study protein expression, interactions, and localization.

HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Invitrogen HA tag antibodies reliably detect recombinant HA tagged proteins, and each antibody is validated for use in …

What is an HA tag? The hemagglutinin (HA) tag is a general epitope tag derived from the HA-molecule and corresponds to amino acids 98-106. It has been widely used in expression vectors.

2023年5月11日 · The HA tag is a 9 amino acid peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged amino acids for an overall negative charge. This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with some success for protein purification as well.