G-Rex® stands for Gas Permeable Rapid Expansion and is the result of applying our disruptive technology to the needs of immunotherapy. G-Rex® is the only production platform created specifically to produce immune cells such as T cells, natural killer cells, and hematopoietic cells.

In addition, TIL grown in GRex plates showed a higher percentage of CD8+ cells (94±8% versus 76±14%, p=<0.001) and lower percentage of NK cells (2.4±3% versus 12±9%, p=<0.001) than those grown in polystyrene. Tumor‐ specific activity was comparable, as measured by IFN‐ γ secretion between the two culture conditions.

免疫细胞治疗的方法是采取患者自身的免疫细胞，在体外进行改造、活化及扩增，再回输到患者体内。此方法对人体伤害小且能有效杀伤肿瘤，是治疗肿瘤的有效方法。常用于细胞免疫治疗的细胞包括： car-t 、 tcr-t 、 til 、 nk 、 cik 等。然而，与淋巴细胞的分离 ...

2022年1月19日 · “Clinically relevant levels of transgene expression and cell numbers can be achieved by combining reagents in the GRex, without complicated time-consuming coating steps of traditional transduction.” ... We are thus postulating that the high spare respiratory capacity and the high OCR that we observed in TIL grown in high oxygen environment ...

TIL polyfunctionality was analyzed using flow cytometry at the end of REP following 6-hour activation with anti-CD3 and anti-CD28. The charts show percentage of TIL ... in a 24-well Grex. Total live cell counts measured every 3 days. B. Post-REP TIL from a CRC donor were adoptively transferred to NSG mice dosed daily ...

但TIL细胞的制备过程非常困难。在此，我们使用一种透气型培养瓶建立了快速扩增TIL细胞的简单方法。首先在G-Rex10中培养从肿瘤消化液和肿瘤碎片得到的TIL细胞，接下来使用能容纳500ml培养基的G-REX100进行快速的扩增培养。

We next compared the growth of TIL in G-Rex10 flasks or 24-well plates from tumor fragments. For each tumor sample, fragments approximately 1 to 8 mm 3 in size were seeded into 24-well plates at 1 piece per well and into G-Rex10 flasks at 5, 10, 20 or 30 pieces per flask. A total of 11 tumor samples collected from 9 patients were tested; 3 samples were from 1 …

Our center performs experimental clinical studies with advanced therapy medicinal products (ATMPs) based on polyclonal T cells, all of which are currently expanded in standard T-flasks. Given the need to increase the efficiency and safety of large-scale T cell expansion for clinical use, we have opt …

Rings Advantages of G-Rex ® Bioreactors G-Rex® Bioreactor M-Series Design Closed-system bioreactor for cell production. Scale Intelligently and Successfully G-Rex® is the only cell culture bioreactor equipped to meet current and future patient demand for immune cell therapies.

The aim of this study was to develop methods for the expansion of tumor-reactive TIL from resected soft tissue sarcoma to a degree required for the ACT. ... initial TIL cultures were expanded using a REP developed and employed on prior melanoma clinical trials. 17,31 TIL were loaded into GREX-10 flasks (Wilson-Wolf, St. Paul, MN) with media ...