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Extension of these primers by the telomerase in the presence of 32 P-dGTP and ddTTP, gave rise to relatively abundant elongation products containing 5 newly added nucleotide residues, of which the product GGGGTTg-3' was the most abundant. A study of the time course of accumulation of this product showed that after reaching a maximal level, its ...

Cooper et al. (2019) reported CSB-1 to be 100% conserved (GGGCGTGCA), and CSB-3 to have a major variant (GGGGTTG G TGTA) and a minor variant (GGGGTTG A TGTA) in three minicircles. (Note the first 100 bp of the conserved sequence region, starting with the CSB-1 motif, were not counted toward the 95% identity threshold used for defining ...

2001年8月1日 · We show that overhang length and the identity of the 3′‐terminal nucleotide are tightly regulated. The majority of overhangs terminate in the sequence 5′‐TTGGGGT and >80% are either 14–15 or 20–21 nucleotides in length. No significant changes in overhang length were detected as cells traversed the cell cycle.

2019年12月12日 · Our study confirms these observations on minicircle structure, refines some characteristics and introduces some new features. As expected, CSB-1 was 100% conserved, but three minicircles classes contained a slight variation of CSB-3, GGGGTTG A TGTA.

2022年10月20日 · Using Next-Generation Sequencing of total cellular DNA extracts, we assembled and annotated the kinetoplast genome and investigated minicircle sequence diversity in 38 animal- and human-infective trypanosome strains.

2021年9月28日 · Here, we present a new analysis toolkit—rKOMICS—to streamline the analyses of minicircle sequence diversity in population-scale genome projects. rKOMICS is a user-friendly R package that has simple installation requirements and that is applicable to all 27 trypanosomatid genera.

1994年10月1日 · Trypanosomatids are characterized by the presence of kinetoplast DNA (kDNA), a peculiar form of mitochondrial DNA that consists of several thousand minicircles and a few dozen maxicircles catenated in a network. Within a species, the minicircles are known to differ in nucleotide sequence, but are homogeneous in size and always cross-hybridize.