2016年9月14日 · Here, we report an essential role for TRIF in STING signaling: various activators of STING could not induce genes in the absence of TRIF. TRIF and STING interacted directly, through their carboxy-terminal domains, to promote STING dimerization, intermembrane translocation, and signaling.

(A) The 3C protein associates with TRIF and induces TRIF cleavage. 293T cells were transfected with plasmids encoding Flag-TRIF (lanes 3 and 4), GFP (lanes 1 and 3), or GFP-3C (lanes 2 and 4). The total amount of DNA was kept constant with an empty vector. At 24 h after transfection, cell lysates were immunoprecipitated with anti-Flag antibody.

2017年8月8日 · Unexpectedly, we found that deficiency in TIR domain–containing adaptor-inducing interferon-β (TRIF), a cytosolic adaptor that transduces some TLR signals, worsened hepatic steatosis induced by a high-fat diet (HFD) and that …

2017年9月12日 · Confocal microscopy showed that TRIM32 promoted colocalization of TRIF and TAX1BP1 in certain aggregates which are positive for the autophagosome marker GFP-LC3 or the lysosome marker GFP-LAMP1, while TRIM32-deficiency abolished endogenous association of TRIF with TAX1BP1 induced by poly(I:C).

2008年3月28日 · TRIF is the principal adaptor molecule in LPS-induced IFNβ production. We examined the subcellular distribution of TRIF during LPS response. Overexpression of TRIF was reported to induce apoptosis due to the conserved residues VQLG in a receptor interacting protein homotypic interaction motif (RHIM) [16]. The VQLG sequence was, therefore ...

TRIF-related adaptor molecule (TRAM) is the fourth Toll/IL-1 resistance domain-containing adaptor to be described that participates in Toll-like receptor (TLR) signaling. TRAM functions exclusively in the TLR4 pathway.

2008年11月28日 · We found that MyD88 or Trif expression led to GFP-LC3 dots in 60–70% of the GFP-LC3 positive cells, nearly similar to that achieved by the overexpression of Beclin 1. We also expressed the TIR-domain containing adapters SARM …

The levels of IFN-γ and Th1 cell-promoting cytokines, including IL-12 p40 and IL-18, were significantly up-regulated 72 h after immunization with pGA-GFP-TRIF compared to control groups (Fig. (Fig.3) 3) (P < 0.05). Th2 cytokine mRNAs, including IL-4 and IL-6, were also up-regulated at 72 h (P < 0.05).

2023年1月13日 · In teleost, mandarin fish SARM could interact with MyD88 and TRIF to impair the host antiviral signaling , and grass carp SARM could down-regulate the mRNA expression level of immune-related genes during the TRIF- and MyD88-mediated signaling pathways, thus suppressing type I IFN production in response to GCRV infection .

2016年9月14日 · Here, we report an essential role for TRIF in STING signaling: various activators of STING could not induce genes in the absence of TRIF. TRIF and STING interacted directly, through their carboxy-terminal domains, to promote STING dimerization, intermembrane translocation, and signaling.