2022年3月1日 · Gamma radiation is credited for the Hulk’s green glow, but has never actually been reported to turn skin green. Green-glowing animals have been genetically engineered by the introduction and expression of the gene for the Green Fluorescent Protein (GFP) and observed under blue to ultraviolet light.

Whenever Banner got angry or went into a rage, the artificial steroids changed him from an ordinary man into the gigantic, dull-witted Hulk, and the steroids activated the GFP gene in Banner's body, causing his skin to glow green.

2009年6月23日 · GFP has also entered the art world with Eduardo Kac's GFP bunny Alba and even the movies as evident by the opening credits of the film Hulk by director Ang Lee that was released in 2003. The implication is that the Hulk is the first human GFP transgenic.

Superfolder GFP整合了9个点突变：S30R、Y39N、F99S、N105T、Y145F、M153T、V163A、I171V以及A260V。相比于野生型的GFP，Superfolder GFP具有更好的稳定性及更高效的折叠动力学（能在37 ℃条件下高效折叠），亮度也比EGFP要高，同时表现出相比与GFP家族其他成员更强 …

2021年7月8日 · (A) Schematic depicting Hulk plasmid encoding green fluorescent protein (GFP), 2W1S peptide, and FLAG peptide under control of the Strongyloides stercoralis actin (Ss-act-2) promoter. (B) Brightfield DIC (top) and green fluorescent (bottom) images of the first and third larval stages, adult free-living female stage, and egg stage of Hulk (L1 ...

2018年1月25日 · One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 ± 0.2 g/l GFP-HFBI in suspension and kept its high performance during prolonged subculturing. In addition, both cultures were successfully cryopreserved enabling truly industrial application of …

绿色荧光蛋白 (green fluorescent protein, GFP) 是当今生命科学和医学研究中被广泛使用的一种示踪物。它使人们可以在活体中直接观察到从微观到宏观各个层次上的丰富多彩的生命现象。做生物学研究的小伙伴想必都知道GFP，但很多人可能不知道或经常搞错其发现者。

2022年6月17日 · （A）烟草BY-2 Hulk细胞中积累了GFP-HFBI蛋白；（B）对培养的蛋白进行含量测定；（C）SDS-PAGE检测蛋白；（D、E）鲜重、干重的测定；（F）共聚焦显微镜显示GFP-HFBI在蛋白质体中的积累。 4.2.2.3 原生质体. 利用原生质体转化目的基因是最为常见的瞬时转化体系之一，尤其是应用于蛋白定位及蛋白互作鉴定等方面。 如果某物种无转化体系，可以尝试建立该物种的原生质体瞬时转化体系，将其愈伤、悬浮细胞、叶片或茎等经过酶解制作原生质体。

In popular culture, GFP was evoked in the Ang Lee movie Hulk to explain why this superhero has green skin. One of the real joys of having introduced a new technology is being astonished by the remarkable new and imaginative uses that other people devise.

To assess the subcellular protein localization of HULK gene products, we transiently expressed and visualized GFP/YFP-tagged proteins in tobacco epidermal cells (Nicotiana benthamiana). All four HULK proteins localized to the nucleus (Figure 1 c), a finding consistent with the presence of NLS motifs in each family member (Figure 1 a).