In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre- lox recombination but involves the recombination of sequences between short flippase recognition target ( FRT ) sites by the recombinase flippase ( Flp ...

2010年1月17日 · We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell.

2011年10月20日 · Flowcell reverse transcription sequencing (FRT-seq) 18 is an RNA-seq approach for Illumina sequencers, which combines a paired-end library preparation...

We report an alternative approach to transcriptome sequencing for the illumina Genome analyzer, in which the reverse transcription reaction takes place on the flowcell. no amplification is...

针对mRNA-seq，近日Lira Mamanova团队以Illumina测序平台为基础，发展出可直接以RNA library做为测序材料的新技术，名为FRT-seq (Flowcell Reverse Transcription sequencing)，此技术将RNA library注入flowcell内，并且直接在flowcell内执行反转录及bridge PCR，FRT-seq能完整保留RNA正负股的信息 (即strand-specific mRNA sequencing)，除此之外，其制备library过程中不需经由PCR的放大，所以可以减少PCR所产生的错误。

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We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is …

Flow-cell surface reverse transcription sequencing (FRT-Seq) is an transcriptome sequencing technique developed in 2010 by Mamanova et al. The method is strand-specific, free of amplification, and compatible with paired-end sequencing. To begin with, poly(A) RNA samples are fragmented by metal-ion hydrolysis and dephosphorylated.

To understand the distribution and sequence properties of genomic FRT -like sites, we performed a genome-wide analysis of FRT -like sites in the human genome using the experimentally-derived parameters. Out of 642,151 identified FRT -like sequences, 581,157 sequences were unique and 12,452 sequences had at least one exact duplicate.

Flowcell Reverse Transcription Sequencing. Flow-cell surface reverse transcription sequencing (FRT-Seq) is an transcriptome sequencing technique developed in 2010 by Mamanova et al (Mamanova et al., 2010). The method is strand-specific, free of amplification, and compatible with paired-end sequencing.