FluoZin™-3 指示剂是一种具有与 fluo-4 相似结构的 Zn 2+ 选择性指示剂。FluoZin™-3 表现出高 Zn 2+ 结合亲和力，不受高达至少 1 µM Ca 2+ 浓度影响。此外，在 Zn 2+ 饱和水平下，FluoZin™-3 显示荧光增加了 >50 倍。尽管以 FluoZin™-3 的成像应用为主，但也开发了基于细胞的 ...

FluoZin™-3 indicator is available in a cell-permeant and a cell-impermeant form. We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more.

2018年10月9日 · Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn 2+ probe and affords uniform measurement of resting Zn 2+ levels at the single cell level with proper calibration. In...

The FluoZin™-3 indicator is a Zn 2+-selective indicator with a structure that resembles fluo-4. FluoZin™-3 exhibits high Zn 2+-binding affinity that is unperturbed by Ca 2+ concentrations up to at least 1 μM. In addition, FluoZin™-3 exhibits a >50-fold increase in fluorescence in response to saturating levels of Zn 2+. Although imaging ...

Although imaging applications of FluoZin-3 are predominant, cell-based microplate assays and flow cytometry protocols have also been developed; Label (Ex/Em): FluoZin-3 (approximately 494/516nm) K d (Zn 2+) (in buffer): approximately 15 nM; Lyophilized product may be dissolved in …

FluoZin™-3 (K d (Zn 2+) ~ 15 nM) is suitable for detection of Zn concentrations in the 1–100 nM range and it has shown to be the most Zn2+-sensitive and Zn2+-specific of the three FluoZin™ Zn2+ indicators. The cell permeant AM-ester is useful for detecting low intracellular Zn2+ levels and small concentration changes.19

FluoZin™ indicators are offered in cell-permeant and cell-impermeant forms. We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more.

FluoZin-3 is a member of the class of xanthenes that is used as a Zn(2+)-selective fluorescent indicator. It has a role as a histological dye, a chelator and a visual indicator. It is a member of xanthenes, a cyclic ketone, an aromatic ether, a member of phenols, an organofluorine compound, a tricarboxylic acid and a substituted aniline.

FluoZin-3 has been found to be a brighter alternative to Zinquin for measuring the exocytotic release of Zn 2+ from pancreatic β-cells after stimulation with glucose or potassium. Although imaging applications of FluoZin-3 are predominant, cell-based microplate assays and flow cytometry protocols have also been developed.

2020年9月18日 · We present a protocol to measure changes in cellular zinc concentrations using either a low-affinity membrane permeable or a high-affinity membrane impermeable fluorescent dye. Overall, zinc-specific fluorescent indicators using the assay can reliably detect the Zn 2+ flux into or out of cultured cells.