2024年5月22日 · We investigated oxidation of flavin by molecular oxygen, which is a highly relevant reaction involved in many life processes because a number of enzymes use flavin as a cofactor, mainly in oxidoreductive reactions. In such enzymes, flavin oxidation is required to restore the catalytic capabilities of an enzyme, thus completing the catalytic cycle.

2018年1月11日 · The oxidation of free flavin proceeds with an estimated rate of 250 M –1 s –1, leading to the accumulation of reactive species, in particular flavin radicals and O 2 –•. Thorough kinetic and chemical analyses suggest an initial electron transfer from singlet reduced flavin to triplet O 2 to yield a caged radical pair.

2020年1月1日 · Flavin oxidase and dehydrogenase catalyze the oxidation of main substrates in their reductive half-reaction, while the reduced flavin is reoxidized in another half-reaction by transferring electrons to molecular oxygen (oxidase) or to other organic molecules such as NAD(P) + or benzoquinone derivatives (dehydrogenase).

2013年10月27日 · We show that EncM maintains an unexpected stable flavin-oxygenating species, proposed to be a flavin-N5-oxide, to promote substrate oxidation and trigger a rare …

Flavin adenine dinucleotide (FAD)-dependent monooxygenases (FMOs) catalyze many oxidation reactions with chemo-, regio-, and stereo-selectivity, and they are involved in the synthesis of many natural products. In this review, we introduce the mechanisms for different FMOs, with the classical FAD (C4a)-hydroperoxide as the major oxidant.

Flavin-N5-oxide, a recently discovered flavin oxidation state, is formed by two-electron oxidation of flavin or by four-electron oxidation of reduced flavin. This novel oxidation state was first observed in the EncM-catalyzed 1,3-diketone oxidation in enterocin biosynthesis [3–6].

2012年9月1日 · Oxidation of reduced flavin by O 2 is one of the most fascinating reactions in biochemistry. Flavins [generally in the form of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN); see Glossary] are versatile redox cofactors, capable of receiving up to two electrons from reducing substrates and conveying them to electron acceptors [1].

2024年5月22日 · As a highly electrophilic moiety capable of oxidizing a variety of small organic molecules and biomolecules, flavin is an important prosthetic group in many enzymes. Upon oxidation of the substrate, flavin is converted into its reduced (dihydrogenated) form. The catalytic cycle is completed through …

2020年4月22日 · Oxygen activation mechanisms catalyzed by flavin cofactors are established for electron transfer (oxidases) and C4a covalent flavin–oxygen adduct formation (oxygen-inserting monooxygenases). A...

2013年5月3日 · This review provides an overview of oxidases that utilise a flavin cofactor for catalysis. This class of oxidative flavoenzymes has shown to harbour a large number of biotechnologically interesting enzymes. Applications range from their use as biocatalysts for the synthesis of pharmaceutical compounds to the integration in biosensors.