First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC).

Fluorescein (FITC) derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water-solubility.

FITC is a fluorescein derivative consisting of a fluorescein core and an amine-reactive isothiocyanate moiety. The isothiocyanate group reacts with primary amines forming covalent thiourea bonds that attach fluorescein to the biomolecule.

FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry.

2009年4月18日 · Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Fluorescein isothiocyanate (FITC) is a fluorescent probe that is commonly used in the preparation of conjugates with biological molecules. It has high water solubility, bright fluorescence, and low nonspecific binding with biological tissues. FITC can be excited by blue light and emits green to yellow fluorescence.

2022年3月17日 · First termed in 1942 by A.H. Coons, FITC is a unique fluorescein molecule having an isothiocyanate reactive group (-N=C=S), which replaced the hydrogen atom located at the base of its structure. In this article, we’ll discuss FITC labeling techniques and provide tips and tricks for maximizing yield.

FITC fluorescein isothiocyanate | C42H22N2O10S2 | CID 131730176 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines.

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as …