Similar to the wild-type ferrochelatase, four variants exhibited the electronic absorption features characteristic of [2Fe-2S] cluster-containing proteins, including absorption bands at 330...

1996年2月27日 · UV-visible absorption spectroscopy of both human and murine recombinant ferrochelatase incubated with NO or the NO donor, S-nitroso-N-acetylpenicillamine (SNAP), indicate a rapid loss of the visible absorption spectrum of the [2Fe-2S] cluster.

1994年1月18日 · The UV-visible absorption spectrum of the purified enzyme consists of a protein absorption at 278 nm (epsilon approximately 90,000 M-1 cm-1) and bands at 330 nm (epsilon approximately 24,000 M-1 cm-1), 460 nm (shoulder, epsilon approximately 11,000 M-1 cm-1), and 550 nm (shoulder, epsilon approximately 9000 M-1 cm-1) that are indicative of a ...

2001年12月25日 · A previous study revealed that recombinant ferrochelatase, as well as purified bovine ferrochelatase, showed an absorption spectrum similar to the Fe/S cluster.

UV–vis electronic absorption spectra were recorded using a 1 mm cuvette or a 5 mm NMR tube at 25°C by means of a Cary 60 spectrophotometer (Agilent Technologies, Santa Clara, CA) with a scan rate of 600 nm min −1 and a resolution of 1.5 nm. All the spectra were normalized to the intensity of the Soret band and the 450–700 nm region has ...

2000年2月18日 · Ferrochelatase (EC 4.99.1.1), the terminal enzyme of the heme biosynthetic pathway, catalyzes Fe 2+ chelation into protoporphyrin IX. Resonance Raman and UV−vis absorption spectroscopies of wild-type and engineered variants of murine ferrochelatase were used to examine the proposed structural mechanism for iron insertion into porphyrin.

2023年9月24日 · Coproporphyrin ferrochelatase plays an important physiological role as the metalation represents the penultimate reaction step in the prokaryotic coproporphyrin-dependent heme biosynthetic pathway, yielding coproheme (ferric coproporphyrin III).

2022年6月1日 · A bis-histidine coordination for Fe 2+ or Co 2+ was also supported with the X-ray absorption spectroscopic studies using S. cerevisiae ferrochelatase reacted with either Fe 2+ or Co 2+ and murine ferrochelatase reacted with Co 2+ [118].

1982年12月25日 · Ferrochelatase (protohaem ferro-lyase EC 4.99.1.1) has been purified to apparent homogeneity from the facultative photosynthetic bacterium Rhodopseudomonas sphaeroides. The enzyme has been purified 1,640-fold with 43% recovery from isolated membrane fragments.

1986年1月1日 · The absorption spectrum of the R. sphaeroides enzyme has a maximum at 278 nm with no absorption in the visible light range. The ferrochelatase of both R. sphaeroides and A. itersonii has similar kinetic parameters and sensitivities to various metal ions.