2017年9月26日 · Escherichia coli cells have three formate dehydrogenases (FDHs), FDH-H, FDN-N and FDH-O. All three FDHs contain selenocysteine and the molybdenum (Mo) cofactor, which are required for their formate dehydrogenase activities. FDH-N complex and FDH-O complex are localized in inner membrane, oxidize formate …

1997年6月24日 · By in vitro selection, novel RNA sequences ("aptamers"), which can interact tightly and specifically with SelB, were isolated from an RNA library. The library was comprised of mutagenized variants of the wild-type fdhF mRNA hairpin. One-half of the selected sequences contained the apical stem-loop of the fdhF mRNA hairpin highly conserved.

2014年8月25日 · In this work, genetic tools are reported that allow the facile isolation of FHL in a single chromatographic step. The core complex is shown to comprise HycE (a [NiFe] hydrogenase component termed Hyd-3), FdhF (the molybdenum-dependent formate dehydrogenase-H), and three iron-sulfur proteins: HycB, HycF, and HycG.

2017年5月10日 · FHL comprises a formate dehydrogenase (encoded by fdhF) linked directly to [NiFe]-hydrogenase-3 (Hyd-3), and formate is the only natural substrate known for proton reduction by this hydrogenase. In this work, the possibility of engineering an alternative electron donor for hydrogen production has been explored.

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Analogous mutants, termed fdhD and fdhE, have been isolated in Escherichia coli. We used gene fusions between fdnG, the structural gene for the large subunit of formate dehydrogenase-N, and lacZ, the structural gene for beta-galactosidase, to examine E. coli fdnGHI operon expression in fdhD and fdhE insertion mutants.

2011年11月23日 · IscS is one of the three Escherichia colil -cysteine desulfurases. It interacts with FdhD, a protein essential for the activity of formate dehydrogenases (FDHs), which are iron/molybdenum/selenium-containing enzymes. Here, we address the role played by this interaction in the activity of FDH-H (FdhF) in E. coli.

Formate is an important and widespread electron donor available to bacteria in anaerobic environments. During anaerobic growth with nitrate, E. coli can express a membrane-bound formate dehydrogenase (FdhN), which oxidizes formate in the periplasmic compartment and for which a high-resolution structure has been determined.

2008年2月26日 · Repressed by oxygen, nitrate, nitrite, and other electron acceptors. Consists of two separable enzymatic activities: a formate dehydrogenase component (FDH-H) and …

2014年4月1日 · The MVAD Pump (HeartWare Inc, Framingham, MA) is a small, axial VAD that uses magnetic and hydrodynamic impeller technology and incorporates wide helical flow channels to minimize shear stress. In this study, we implanted the MVAD Pump in an ovine model to evaluate device hemocomaptiblity, biocompatibility, performance, and safety.