Phosphofructokinase-2 (6-phosphofructo-2-kinase, PFK-2) or fructose bisphosphatase-2 (FBPase-2), is an enzyme indirectly responsible for regulating the rates of glycolysis and gluconeogenesis in cells.

2008年4月1日 · We down-regulated the endogenous isoforms of PFK-2/FBPase-2 with siRNA (small interfering RNA) and expressed KA (kinase active) and KD (kinase deficient) variants to distinguish between the role of PFK-2/FBPase-2 protein and the role of its product, fructose 2,6-P (2), in regulating beta-cell function.

磷酸果糖激酶2 （英语： Phosphofructokinase-2； PFK2； EC 2.7.1.105）是一种生物体内双重功能酶，在某些 细胞 作用，扮演调节血液 葡萄糖 浓度的角色。 磷酸果糖激酶-2与 果糖二磷酸酶-2 （FBPase2）两者是存在于一条多肽链上的两个酶，这个蛋白质被磷酸化后，磷酸果糖激酶-2的催化水平下降而果糖二磷酸酶-2的催化水平上升，于是 果糖-2,6-二磷酸 在细胞中的水平下降。

Fructose-2,6-bisphosphate is responsible for mediating glucagon-stimulated gluconeogenesis in the liver. This discovery has led to the realization that this compound plays a significant role in directing carbohydrate fluxes in all eukaryotes.

Here we show that treatment of rat hepatocytes with 25mM glucose caused decreased binding of glucokinase to GKRP, translocation from the nucleus and increased binding to 6-phosphofructo 2-kinase/fructose 2,6 bisphosphatase-2 (PFK2/FBPase2) in the cytoplasm.

2001年1月1日 · PFK-2/FBPase-2 has many of these properties, making it responsive to a plethora of metabolic and hormonal signals. This is consistent with its central role in regulation of carbohydrate metabolic fluxes in a diverse cross-section of tissue types and eukaryotic life-forms.

Since its discovery more than 20 years ago, inroads have been made towards the understanding of the structure–function relationships in PFK-2 (6-phosphofructo-2-kinase)/FBPase-2 (fructose-2,6-bisphosphatase), the homodimeric bifunctional enzyme that catalyses the synthesis and degradation of Fru-2,6-P 2.

2001年1月1日 · We have attempted to show that since its discovery in the early 1980s, the study of PFK-2/FBPase-2 has led to a significant expansion in the number of genes and isozymic gene products, as well as an increase in structural and regulatory (both acute and long-term) information about this enzyme.

2022年11月20日 · Our results showed that the M. thermophila genome encodes three isomers, all with the PFK2/FBPase-2 structure: pfk2-a, pfk2-b, and pfk2-c. Overexpression of each gene revealed that endogenous expression of pfk2-c (PFK2 activity) promoted glucose metabolism, while overexpression of pfk2-a (FBPase-2 activity) inhibited strain growth.

磷酸果糖激酶2 （英语：Phosphofructokinase-2； PFK2； EC 2.7.1.105）是一种生物体内双重功能酶，在某些 细胞 作用，扮演调节 血液 葡萄糖 浓度的角色。 磷酸果糖激酶 -2与 果糖 二磷酸酶 -2 （FBPase2）两者是存在于一条 多肽链 上的两个酶，这个 蛋白质 被 磷酸化 后，磷酸果糖激酶-2的 催化 水平下降而果糖二磷酸酶-2的催化水平上升，于是果糖-2,6- 二磷酸 在细胞中的水平下降。