Tet-Off系统包括两个基因片段。 片段一，由一个启动子和tTA（tetracycline-controlled transactivator，四环素控制的激活因子）基因组成。 以即刻早期基因c-fos的启动子为例。 当 …

These mice allow the quantification of c-fos expression (neuronal activation) by measuring shEGFP fluorescence, and are also a Tet-Off tool that allows dox-conditional expression of a …

2019年11月12日 · (a) The c-fos-driven expression of tetracycline-controlled transactivator (tTA) induces Cre expression and Cre-dependent expression of tdTomato, which can be blocked by …

包含Fos (小鼠骨肉瘤致癌基因FBJ的最小启动子)驱动的四环素响应转录激活因子 (tTA)序列、SV40的poly (A)增强子序列，以及另一个构建，其中包含Fos最小启动子驱动的、半衰期为两 …

Plasmid fos-tTA from Dr. Mark Mayford's lab contains the insert Fos promoter/first intron -764/+918 and is published in Science. 2007 Aug 31;317 (5842):1230-3. This plasmid is …

2007年8月31日 · The TetTag mouse combines elements of the tetracycline-transactivator (tTA) system for transgene regulation with neuronal activity–induced activation of the c-fos promoter …

2024年12月17日 · One common approach to labeling these cells is to use the fos-tTA transgenic mouse (TetTag). In addition to tagging cells active during learning, it is common to examine …

008344 B6;DBA-Tg (Fos-tTA,Fos-EGFP*)1Mmay Tg (tetO-lacZ,tTA*)1Mmay/J The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of …

这个c-fos-tTA转基因是通过将小鼠FBJ骨肉瘤致癌基因（Fos或c-fos，包含至少611bp的5'非转录区，包含已知的c-fos调控元件）的启动子区域置于tetracycline调控的转录激活器基因（tTA …

2017年9月11日 · For example, the c-Fos-tTA mouse 5 bears a Fos promoter coupled to the tetracycline-transactivator (tTA), a key component of the doxycycline system for inducible …