In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo.

利用Cre/LoxP和来自酵母的Flp/FRT系统可以研究特定组织器官或特定细胞中靶基因灭活所导致的表型。 通过常规基因打靶在基因组的靶位点上装上两个同向排列的loxp，并以此两侧装接上loxp的（loxP floxed）ES细胞产生“loxP floxed”小鼠。

2019年2月13日 · FLP是一个重组酶（flippase，Flp），类似于Cre，它识别的位点是FRT，当FRT在同一条DNA上且方向相同时，则两FRT位点之间的基因则被删除；FRT方向相反，则基因被倒转。

2006年5月19日 · Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene expression to be closely regulated. This article briefly outlines …

2012年1月1日 · Cre and FLP recombinases are the most widely used site-specific recombinases in genome engineering. Both are members of the tyrosine class of recombinases and catalyze the reversible, site-specific recombination between two identical sequences of 34 bp length in the absence of accessory factors.

Cre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed.

We present a two-part system for conditional FLP-out of FRT-flanked sequences in Caenorhabditis elegans to control gene activity in a spatially and/or temporally regulated manner. Using reporters, we assess the system for efficacy and …

In the Flp-In System, three different vectors are used to generate isogenic stable mammalian cells lines expressing your gene (s) of interest. The first major component of the Flp-In™ System is the pFRT/lacZeo target site vector that is used to generate a Flp-In host cell line.

2020年10月15日 · Flp-TAL recombinases are hybrid enzymes that are composed of two functional modules: a variant of site-specific tyrosine recombinase Flp, which can have either narrow or broad...

In this study, the FLP/LoxP-FRT recombination system was used as a gene switch, controlling the temporal and spatial specificity to precisely regulate target gene expression. The FLP recombination acted as the “gene key” while Nos terminator was the “gene lock” that hindered the expression of eGFP .