2020年2月1日 · Here, a padlock probe (PLP)-based rolling circle amplification (RCA) method for the detection of EBOV is presented. For this, a set of PLPs, separately targeting the viral RNA and complementary RNA of all seven EBOV genes, was used in the RCA assay and validated on virus isolates from cell culture.

2016年5月1日 · A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity.

EBOV is defined by a fast spread by exchange of body fluids or by close contact with infected persons, three weeks incubation periods and rapid death within 4 to 10 days from infection [28].

5 天之前 · In this study, we modeled and inserted four overlapping fragments (F1–4) of the EBOV GP at the C-terminus of the A 1 protein of Qubevirus (Qβ) and used the platform to investigate the tropism and immunological functions of the GP by displaying the …

2023年9月21日 · 研究人员以埃博拉病毒（EBOV）L聚合酶作为研究对象，发现了新合成的L聚合酶的复制过程受EBOV基因组特异性的3'引导序列的控制，确定了EBOV L-VP35-RNA复合物的高分辨率结构，证实了RNA的弯曲构象对于新合成RNA活性是必需的，阐明了稳定RNA构象的关键L 蛋 …

2018年4月3日 · Here, we report that the structural features of ebolavirus envelope glycoproteins (GPs) and one of their counter receptors, macrophage galactose-type calcium-type lectin (MGL/CD301), play crucial...

2020年2月20日 · Ebola virus disease (EVD) is a severe and frequently lethal disease caused by Ebola virus (EBOV). EVD outbreaks typically start from a single case of probable zoonotic...

2017年10月27日 · Quantification of viral RNA (vRNA), cRNA, and mRNA levels of the EBOV minigenome demonstrated that the stable EBOV replicon had much-more-active minigenome replication than previously developed transient-transfection-based EBOV minigenome systems, which recapitulate viral primary transcription more than genome replication.

2017年11月21日 · Understanding how infected cells respond to Ebola virus (EBOV) and how this response changes during the process of viral replication and transcription are very important for establishing effective antiviral strategies. In this study, we conducted a genome-wide screen to identify long non-coding RNAs …

2016年5月1日 · A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of ...