2023年8月24日 · Cytoplasmic dynein-1 transports intracellular cargo towards microtubule minus ends. Dynein is autoinhibited and undergoes conformational changes to form an active complex that consists of...

Dynein transports various cellular cargos, provides forces and displacements important in mitosis, and drives the beat of eukaryotic cilia and flagella. All of these functions rely on dynein's ability to move towards the minus-end of the microtubules, known as retrograde transport; thus, they are called "minus-end directed motors".

2018年4月16日 · Cytoplasmic dynein 1 is an important microtubule-based motor in many eukaryotic cells. Dynein has critical roles both in interphase and during cell division. Here, we focus on interphase...

Cytoplasmic dynein-1 (dynein) is a microtubule motor required for fundamental processes such as intracellular trafficking, organelle positioning, and the function of the mitotic spindle. Dynein activation requires the assembly of a ~4-MDa complex with its cofactor dynactin and a cargo-specific adaptor protein.

2020年11月10日 · We propose that a tunable LIC1-adaptor interaction modulates dynein’s motility in a cargo-specific manner. In eukaryotic cells, a single motor, cytoplasmic dynein-1 (dynein), carries out the...

In neuronal axons, dynein-1 is the only motor that drives retrograde transport toward the cell body (125, 148). Dynein-1 also plays important roles in mitosis, including positioning the spindle, focusing the MTs into poles, and regulating the spindle assembly checkpoint (152).

2023年12月18日 · Cytoplasmic dynein (dynein-1) is responsible for transporting intracellular cargos in the retrograde direction in the cytoplasm, anchoring several organelles to the microtubule network, driving nuclear migration in developing neurons, and orienting the mitotic spindle in dividing cells.

2021年5月20日 · Dynein is a multi-subunit nanomotor built around a core of large dimeric heavy chains (HCs), which perform ATP hydrolysis to power motion and provide a molecular scaffold for assembling the intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs) into the dynein holocomplex (Pfister et al., 2006).

Here, we present a cryoelectron microscopy structure of the complete 1.4-megadalton human dynein-1 complex in an inhibited state known as the phi-particle. We reveal the 3D structure of the cargo binding dynein tail and show how self-dimerization of the motor domains locks them in a conformation with low microtubule affinity.

2023年12月18日 · Cytoplasmic dynein (dynein-1) is responsible for transporting intracellular cargos in the retrograde direction in the cytoplasm, anchoring several organelles to the microtubule network, driving nuclear migration in developing neurons, and orienting the mitotic spindle in dividing cells.