差示扫描荧光法（Differential Scanning Fluorimetry, 简称DSF），是在荧光定量PCR仪上缓慢加热样品，在加热的过程中检测荧光染料与结构发生改变的蛋白质相结合的量，来评价蛋白质热稳定性的方法。

2025年1月22日 · 差示扫描荧光法（Differential Scanning Fluorimetry，DSF），又称热迁移分析（Thermal Shift Assay，TSA）或ThermoFluor，是一种成本效益高、可并行化、实用且易于使用的生物物理技术（图1）。因此，它被广泛用作跟踪蛋白质折叠状态和稳定性以及相互作用等研究。

2025年2月7日 · Here, we present a microplate-based intrinsic DSF approach that enables the measurement of up to 384 samples in parallel by consuming only 10 μL per sample. We systematically test and benchmark the new intrinsic DSF against gold-standard methods such as differential scanning microcalorimetry and circular dichroism.

2023年12月15日 · Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, and optimization.

2024年5月14日 · Differential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by fluorogenic...

差示扫描荧光法 (dsf) 可以通过评估随温度升高时蛋白质构象的变化，快速为您筛选出理想的蛋白质和配方选择。 充分利用荧光 差示扫描荧光的发工作原理是：当蛋白质构象发生变化时，通过监测荧光的变化来评估蛋白质的稳定性。

2020年1月31日 · We illustrate the uses of DSF in protein buffer optimization for stability, refolding, and crystallization purposes and provide several examples of each. We also show the use of DSF in a more downstream application, where it is used as an in vivo validation tool of ligand-target interaction in cell assays.

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Intrinsic differential scanning fluorimetry (DSF) is essential for analyzing protein thermal stability. Until now, intrinsic DSF was characterized by medium throughput and high consumable costs. Here, we present a microplate-based intrinsic DSF approach that enables the …

We illustrate the uses of DSF in protein buffer optimization for stability, refolding, and crystallization purposes and provide several examples of each. We also show the use of DSF in a more downstream application, where it is used as an in vivo validation tool of ligand-target interaction in cell assays.