Biotinylated dextran amines (BDA) are organic compounds used as anterograde and retrograde neuroanatomical tracers. They can be used for labeling the source as well as the point of termination of neural connections and therefore to study neural pathways .

The amino 70,000 MW dextran can be conjugated to dyes and other molecules using amine-reactive moieties including succinimidyl esters or isothiocyanates. Dextrans are biologically inert hydrophilic polysaccharides that are used in a wide varie

Biotinylated dextran amine (BDA) —nonfluorescent, aldehyde-fixable dextrans simultaneously labeled with biotin and lysine and available in several molecular weights (D1956, D1957, D7135, D7142). A useful review has been published on the BDA products.

Dextran amines are versatile and sensitive tools for anterograde and retrograde investigation of neural connectivity. Because of their tolerance of diverse fixatives, they are ideal for various light and electron microscopic studies.

The lysine fixable, biotinylated 10,000 MW dextran amine (BDA-10,000 is widely used as a neuronal tracer. It contains free amines and can therefore be fixed in place with formaldehyde or glutaraldehyde. The biotin can be detected using avidin

Biotinylated dextran amine (BDA) is a highly efficient and powerful marker for bidirectional tracing of nerve pathways in a wide variety of species at the light and electron microscopic level. The BDA tract-tracing method can readily be combined with other anterograde or …

2000年11月15日 · Fluorescent dextran amines rely on the transport properties of dextran to allow pathway tracing, with the lysine moiety making it possible to fix the dextran in situ with standard aldehyde fixatives and the fluorophore making it possible to visualize the localization of the dextran amine by fluorescence microscopy.

High molecular weight biotinylated dextran amine (BDA) has been used as a highly sensitive neuroanatomical tracer for many decades. Since the quality of its labeling was affected by various factors, here, we provide a refined protocol for the application of high molecular weight BDA for studying optimal neural labeling in the central nervous ...

The amino 70,000 MW dextran can be conjugated to dyes and other molecules using amine-reactive moieties including succinimidyl esters or isothiocyanates. Dextrans are biologically inert hydrophilic polysaccharides that are used in a wide varie

We therefore evaluated the efficacy of a biotinylated dextran amine (BDA) for anterograde labeling in several different preparations. BDA was visualized with an avidin-biotinylated HRP (ABC) procedure followed by a standard or metal-enhanced diaminobenzidine (DAB) reaction.