The net positive charge of the resulting nucleic acid-DEAE‑dextran complex allows it to adhere to the cell membrane and enter into the cytoplasm via endocytosis or osmotic shock induced by …

DEAE-dextran is a cationic polymer that tightly associates with negatively charged nucleic acids. The positively charged DNA:polymer complex comes into close association with the negatively …

DEAE-dextran可以促使DNA结合到细胞膜上，然后通过内吞作用(endocytosis)进入细胞。 基于DEAE-dextran的细胞转染方法适用于瞬时转染(transient tranfection)，但不适用于筛选稳定表达 …

Chloroquine is thought to enhance transfection efficiencies by neutralizing lysosomal hydrolases, which can degrade the DNA. This protocol describes transfection of lymphocyte cell lines, but …

2019年2月1日 · In this aspect, the DEAE Dextran particles display clearly different transfection characteristics than those formed from cationic lipids, which showed transfection both with …

2009年7月23日 · When comparing transfection efficiency for DEAE-dextran (graft ratio 0%) and DDMC in transfection experiments carried out using HEK293 cells, remarkable results, to have …

The oldest of the transfection methods (initiated as early as the 1960s) uses DEAE-dextran, to direct the DNA into the cells. The DNA is bound to polycations, and the dextran-DNA …

DEAE-dextran-based transfection is cost-effective, highly reproducible, and can yield higher transfection efficiency in specific cells compared with other methods. The transfection with …

2018年7月1日 · DEAE-dextran has commonly been found to more effectively enhance transfection when mixed with the virus nucleic acid before cell inoculation (mixed-inoculum …

The net positive charge of the resulting nucleic acid-DEAE‑dextran complex allows it to adhere to the cell membrane and enter into the cytoplasm via endocytosis or osmotic shock induced by …