In chemistry, a dark quencher (also known as a dark sucker) is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat; while a typical (fluorescent) quencher re-emits much of this energy as light. [1] Dark quenchers are used in molecular biology in conjunction with fluorophores.

为了满足多重qPCR应用，黑洞淬灭剂(Black Hole Quencher, BHQ)便应运而生。 BHQ可在较长的波长下起作用，在可见光谱中具有高效的淬灭能力，与其配对的荧光基团波长选择范围更大。

2012年6月6日 · Dark quenchers are chromophores that primarily relax from the excited state to the ground state nonradiatively (i.e., are dark). As a result, they can serve as acceptors for Förster resonance energy transfer experiments without contributing significantly to background in the donor-emission channel, even at high concentrations.

The sequential derviatization of this trivalent azobenzene derivative with two other nonfluorescent azo dyes (Black Hole Quencher BHQ-1 and BHQ-3) and through effective reactions from the “bioconjugation chemistry” repertoire has led to an universal dark quencher (UDQ).

2015年3月12日 · Here, we describe Si-rhodamine-based NIR dark quenchers (SiNQs), which show broad absorption covering this region. They are nonfluorescent independently of solvent polarity and pH, probably due to free rotation of the bond …

Dark quenchers are dyes with no native fluorescence. Until the last few years, quenchers have typically been a second fluorescent dye, for example, fluorescein as the reporter and rhodamine as the quencher (FAM/TAM probes).

With an absorption maximum of 460 nm, our Dark Quencher BMN-Q460 is particularly suitable for the short-wavelength region and represent an excellent alternative for the quenchers Dabcyl and DDQ I. In addition to use in Molecular Beacons, in which it inhibits fluorescent signals by contact-quenching, BMN-Q460 can be used in various molecular ...

Dark quenchers are non-fluorescent chromophores that can absorb excitation energy from a fluorophore and dissipate it as heat. Quenchers are widely used to synthesize oligonucleotides: hydrolyzable DNA probes, «molecular beacons», and «scorpion» primers.

Dark Quenchers. The quencher need not be a fluorophore, however. A non-fluorescent chromophore can be used that overlaps with the donor’s emission (a dark quencher). In such a case, the transferred energy is dissipated as heat. FRET probes that utilize dark quenchers have a number of advantages over their fluorophore-labeled counterparts.

2013年8月4日 · We describe herein a dark fluorescent quencher that is effective over a wide wavelength range, thereby enabling the construction of a multicolored family of protease substrates.