2023年5月13日 · Our study uncovers an internal regulatory mechanism that switches XPB and XPD activities making them mutually exclusive between NER and transcription initiation. By sequentially coordinating the...

2024年5月28日 · Using cryo-EM, we deciphered one of the most enigmatic steps in XPD helicase action: the active separation of double-stranded DNA (dsDNA) and its stalling upon approaching a DNA interstrand...

在3个 DDR激酶 中， DNA-PK 和 ATM 主要被DNA的双链断裂（DSB）激活，而ATR主要被各种单链损伤激活，参与多种DNA损伤的修复，对于复制细胞的生存能力至关重要。

Our studies demonstrate that triplex-induced double strand breaks (DSBs) can stimulate cells to activate apoptosis both in vitro and in vivo. Although knockdown of XPD did not modulate the extent of triplex-induced DSBs, its depletion resulted in a decrease in triplex-induced apoptosis.

2015年7月1日 · Double-strand breaks (DSB), caused by ROS, ionising radiation, and replication, are repaired by DSB repair. Mismatch repair (MMR) is the main defender against replication errors caused by DNA polymerase.

2010年9月10日 · Here, we reveal a novel role of XPD in TAR, showing that the XPD gene product is required for catalysing this pathway. Furthermore, we show that XPD-deficient cells are proficient in homologous recombination (HR) repair of endonuclease-induced double-strand breaks (DSBs) as well as in the repair of thymidine stalled replication forks.

2014年12月18日 · Surprisingly, DSB formation requires the transcription-coupled nucleotide excision repair (TC-NER) factor Cockayne syndrome group B (CSB), but not the global genome repair protein XPC. These findings reveal an unexpected and potentially deleterious role for TC-NER factors in driving R-loop-induced DNA damage and genome instability.

Since clustered DNA damage is considered the signature of ionizing radiation, we have measured the repair of double strand breaks (DSBs), non-DSB bistranded oxidative clustered DNA lesions (OCDLs) as well as single strand breaks (SSBs) in cells exposed to …

Purpose: Xeroderma Pigmentosum (XP) is a rare, recessive genetic disease associated with photosensitivity, skin cancer proneness, neurological abnormalities and impaired nucleotide excision repair of the UV-induced DNA damage. Less frequently, XP can be associated with sensitivity to ionizing radiation (IR).

Xeroderma pigmentosum group D (XPD) is an important component of DNA repair factor (TFIIH), which bridges the 2 groups. Multiple studies have demonstrated that XPD can modulate cellular proliferation [11, 12], while the effects of XPD in cultured VSMCs remain largely uncharacterized.