2020年10月30日 · DNA end resection is initiated by MRN-CtIP generating short, 3′-single-stranded DNA (ssDNA). This newly generated ssDNA is further elongated by multiple nucleases and DNA helicases, such as EXO1,...

布卢姆综合征 (BLM)解旋酶与外切酶1 (EXO1)和DNA2核酸酶共同催化核小体包被DNA上的千碱基长度DNA切除。 由此产生的ssDNA被RPA快速结合，进一步刺激DNA切除。 作为DNA损伤反应（DDR）的一部分，RPA在切除期间被磷酸化。 值得注意的是，在细胞实验和体外实验中，pRPA通过抑制 BLM解旋酶 来抑制DNA切除。 pRPA抑制BLM在DNA末端的起始，促进固有解旋酶的strand-switch活性。 这些发现 证实了pRPA在DNA切除和DNA损伤反应之间存在一个反 …

2019年9月27日 · Exonuclease 1 (EXO1) is the key molecule responsible for 3′ ssDNA formation of DSB end resection. We revealed that EXO1 is inactivated in G1-phase cells via ubiquitination-mediated...

2020年6月18日 · DNA double-strand break repair by homologous recombination begins with nucleolytic resection of the 5’ DNA strand at the break ends. Long-range resection is catalyzed by EXO1 and BLM-DNA2, which...

Human exonuclease 1 (EXO1), a 5′→3′ exonuclease, contributes to the regulation of the cell cycle checkpoints, replication fork maintenance, and post replicative DNA repair pathways. These processes are required for the resolution of stalled or ...

2010年12月22日 · We show that meiotic DSB resection is dramatically reduced in exo1Δ mutants and test the idea that Exo1-catalyzed resection promotes crossing over by facilitating formation of crossover-specific JMs called double Holliday junctions (dHJs).

During meiosis, the exo1 mutation reduces both DSB processing and crossover frequency and causes nondisjunction of homologous chromosomes. We suggest that Exo1, as well as the Mre11 complex, is involved in the processing of DSB ends and that Exo1 is also involved in crossing over to promote the accurate segregation of chromosomes during meiosis.

2010年2月24日 · Cells depleted of Exo1 show chromosomal instability and hypersensitivity to ionising radiation (IR) exposure. We find that Exo1 accumulates rapidly at DSBs and is required for the recruitment of RPA and Rad51 to sites of DSBs, …

2018年6月19日 · DSB resection requires the Mre11‐Rad50‐Xrs2 (MRX) complex, which promotes the activity of Exo1 nuclease through a poorly understood mechanism. Here, we describe the Mre11‐R10T mutant variant that accelerates DSB resection compared to wild‐type Mre11 by potentiating Exo1‐mediated processing.

2021年12月1日 · Exonuclease 1 (EXO1) can catalyze nucleotide chain excision with its conserved N-terminal domain of 5′ to 3′ exonuclease activity, enabling it to influence diverse biological processes facing the challenges of genotoxic environmental factors such as ionizing radiation.