2021年5月11日 · We combine 3D single-molecule localization microscopy with a low-cost fluorescence labeling strategy that does not denature the DNA, to visualize large portions of single human chromosomes in situ at high resolution.

2024年11月29日 · ChromEMT is a method that selectively enhances the electron density and contrast of DNA and interacting nucleosome particles, enabling chromatin ultrastructure and 3D organization to be imaged...

2020年7月27日 · There is a need for methods that can image chromosomes with genome-wide coverage, as well as greater genomic and optical resolution. We introduce OligoFISSEQ, a suite of three methods that...

2020年3月2日 · We compare and contrast population-based chromosome conformation capture approaches and imaging-based approaches and highlight future challenges. Human DNA is 2 m long. How this DNA undergoes...

2020年11月20日 · A team of scientists at Harvard University has developed a new imaging technology for visualizing organization of chromatin, a substance within a chromosome consisting of DNA and protein, across multiple scales in single cells with high genomic throughput.

2021年8月25日 · Through its enhanced power, super-resolution imaging is poised to reveal new worlds of activity within the genome. What the CGI reveals about the organization of genomes will ultimately contribute to our understanding of a wide variety of phenomena, such as chromosome stability, Wu said.

2020年8月20日 · Here we report an imaging technology for visualizing chromatin organization across multiple scales in single cells with high genomic throughput. First we demonstrate multiplexed imaging of hundreds of genomic loci by sequential hybridization, which allows high-resolution conformation tracing of whole chromosomes.

DNA distribution is visualized within chromosomal details of the centromeric and satellite regions, or in interphase chromatin, with high-resolution (10–15 nm) for comparison with the corresponding secondary electron image representing DNA plus protein.

These methods, including chromosome conformation capture and high-resolution light and electron microscopy, are shedding new light on genome architecture and function. Here, we review the emerging picture of genome organization …

We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method.