DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange/red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a …

DiI即DiIC18(3)，全称为1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate，是最常用的细胞膜荧光探针之一，呈现橙红色荧光。 DiI是一种亲脂性膜染料，进入细胞膜后可以侧向扩散逐渐使整个细胞的细胞膜被染色。

Description: DiI is a lipophilic carbocyanine fluorescent dye with red fluorescence, it can be used to label cell membrane for long-term monitoring of cells. Method: For cell membrane labeling. 1. Before imaging, cells are fixed with 4% paraformaldehyde (4 min). 2. For 3D imaging, stain cells with DiI (5 μM; 6 min) after the cell culture is ...

Prepare stock solutions of lipophilic tracers in dimethyl formamide (DMF), dimethylsulfoxide (DMSO), or ethanol at 1 to 2.5 mg/mL. DMF is preferable to ethanol as a solvent for DiO. Stock solutions can be stored for at least six months without deterioration under the same conditions as the undissolved product.

DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a …

DiI 为橙红色荧光染料，激发/发射波长为 551/569 nM，DiI 被用于正向或逆向示踪各类细胞或组织，荧光信号强度高于 DiO，在进入细胞膜时，其荧光信号会被大幅度放大。 如图 4 所示，Dil 染料标记外泌体，然后与目标细胞共培养。 使用共聚焦显微镜捕捉 HOS 细胞 对 Dil 标记外泌体的摄取，其中被标记的外泌体发出特异性橙红色荧光。 图 4. HOS 和 MG63 细胞摄取红色荧光染料 Dil 标记的 hBMSC-Exos [2] DiD. DiD 为一种红色荧光染料，激发/发射波长为 646/663 nM，DiD 荧 …

DiI、DiO、DiD、DiR染料是一族亲脂性的荧光染料，可以用来染细胞膜和其它脂溶性生物结构。 该系列染料进入细胞膜后，会在整个细胞膜上侧向扩散，在zui佳浓度时可以使整个细胞膜染色。 当其与细胞膜结合后其荧光强度大大增强，有着很高的淬灭常数和激发态寿命。 由于该类染料各具特有的荧光颜色，因此可以用来对活细胞进行多色成像和流式分析。 但值得注意的是：该系列染料能标记细胞膜而不破坏其功能，并且能持续更长时间，但如果发生膜融合则可能会染上其他细 …

细胞膜红色荧光染色试剂盒(DiI)，Cell Plasma Membrane Staining Kit with DiI (Red Fluorescence)是一种以DiI为荧光探针，并提供了染色增强剂、能快速灵敏地对细胞膜进行红色荧光染色标记的试剂盒。

赛默飞DIL染色剂是一种亲脂性膜染料，可横向扩散以染色整个细胞。 它在被膜吸收前荧光较弱，适用于细胞膜标记和研究。 了解更多赛默飞产品信息，立即访问我们的网站。

DiI (DiIC 18 (3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C 18 carbon) hydrocarbon chains into the lipid bilayer. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons.