Cyanine3 (Cy3) is a bright, orange-fluorescent dye with excitation suited for the 532 nm laser line and is used for imaging, flow cytometry and genomics.

Available non-sulfonated dyes incude Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy ® stands for 'cyanine', and the first digit identifies the number of carbon atoms between the indolenine groups. Cy2 which is an oxazole derivative rather than indolenin, is an exception from this rule.

Cy3 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 554 nm and an emission peak at 568 nm. Spectrally its similar to Alexa Fluor™ 555.

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Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λ ex =554, λ em =568).

Cy3 Dye is a fluorescent dye used in 2D DIGE to covalently label proteins in samples for comparison. It allows for quantitative analysis by imaging the Cy3 signal separately and determining the labeling ratio in each spot on the gel.

Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. (See all compounds classified as Fluorescent Dyes.) HMDB is offered to the public as a freely available resource.

2022年12月20日 · Cyanine dye is derived from cyanine, which is where its name derives from: Cy2, Cy3, Cy5, and Cy7. All of them can be bonded to nucleic acids, peptides, and proteins by their reaction groups. Cy3 and Cy5 are dyes with high extinction coefficient, which are especially suitable for intracellular sensitive peptide localization experiments and are ...

Cy3 dye stands as a pivotal tool in the realm of fluorescent labeling, significant not only for its vibrant fluorescence but also for its diverse applications in molecular biology and biochemistry. This article seeks to unravel the complexities surrounding Cy3 dye, from its chemical properties to its practical utility in scientific investigations.

2007年9月20日 · In this work, we report the fluorescence and photophysical properties of Cy3 attached covalently to single-stranded and duplex DNA. Steady-state and time-resolved fluorescence techniques were used to determine fluorescence quantum yields, emission lifetimes, and fluorescence anisotropy decays.