2018年3月7日 · Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

2020年9月17日 · Here, we assessed dosage and delivery of TAM for activation of Cre in immune cell subsets assessed longitudinally and spatially using transgenic mice with ubiquitously expressed Cre/ER and...

2025年3月17日 · CREERT2 encodes a transcriptional regulator that influences gene expression in response to cellular signals. Its protein contains conserved DNA-binding domains that interact with promoter regions of target genes.

CreERT2 is a recombinant fusion protein of the cre DNA recombinase and a mutant ligand-binding domain of the human ER. Similarly, to the endogenous ER, CreERT2 is trapped in the cytosol by heat shock proteins.

CreERT由三个关键字Cre、ER、T组合而成，分别对应于 CreERT 系统中的三个关键物质： Cre 重组酶、雌激素受体（ER，estrogen receptor）和药物他莫昔芬（T，Tamoxifen）。 CreERT系统可被药物他莫昔芬诱导开启。 我们一一来拆解CreERT名字里的关键因素。 Cre是一种识别特异位点的基因重组酶，Cre酶的基因序列来源于P1噬菌体的环化重组酶（c yclization re …

The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg (UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT.

We show that after transfection of cultured cells, split-CreERT2 allows induction of DNA recombination by 4OHT with little activity in the absence of 4OHT.

2016年4月14日 · Mutant Estrogen Receptor (ERT2) ligand-binding domain fusions with Cre recombinase are a key tool for spatio-temporally controlled genetic recombination with the Cre/lox system. CreERT2 is efficiently activated in a concentration-dependent manner by the Tamoxifen metabolite trans-4-OH-Tamoxifen (trans-4-OHT).

These findings establish the Itga8-CreERT2 mouse as an alternative to existing SMC Cre mice for unfettered phenotyping of vascular SMCs after selective gene loss.

Plasmid pCAG-CreERT2 from Dr. Connie Cepko's lab contains the insert Cre-ERT2 fusion protein and is published in Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104 This plasmid is available through Addgene.