2021年9月12日 · In their original theoretical model of chromatography, Martin and Synge divided the chromatographic column into discrete sections, which they called theoretical plates. Within each theoretical plate there is an equilibrium between the solute present in the stationary phase and the solute present in the mobile phase [Martin, A. J. P.; Synge, R ...

2015年9月18日 · What Are Theoretical Plates in HPLC? A chromatographic column does not consist of any physical plates which can be measured from time to time to rate a column’s performance efficiency. It is only a theoretical concept drawing a similarity to the huge fractional distillation columns used in the refining of crude petroleum.

Also known as column efficiency, the number of theoretical plates is a mathematical concept and can be calculated using Equation 4. A capillary column/any chromatography column does not contain anything resembling physical distillation plates or other similar features.

In capillary column chromatography HETP is given by the Golay equation. The concept of theoretical plates or trays applies to other processes as well, such as capillary electrophoresis and some types of adsorption.

Models a chromatographic column as a series of narrow, discrete sections called theoretical plates. Theoretical plate is a term coined by Martin & Synge. It is based on a study in which they imagined that chromatographic columns were analogous to distillation columns and made up or numerous discrete but connected narrow layers or plates.

The concept of the theoretical plate arose from distillation column theory and was borrowed by A J. P. Martin to develop the first elution curve theory for the chromatography column. Neither in a distillation column, nor in a chromatographic column, does equilibrium exist in any part of the distribution system.

2018年11月1日 · The number of theoretical plates, which forms the basis of chromatographic theory, is a key parameter used in all modes of chromatography for measuring column efficiency. It is simply the ratio of retention squared to peak variance, a …

Theoretical plates, also known as theoretical stages, are an imaginary construct used to represent the hypothetical separation steps within a chromatographic column. Each theoretical plate represents a stage where equilibrium between the stationary phase and the mobile phase is …

Plate Theory Models a chromatographic column as a series of narrow, discrete sections called theoretical plates. Assume that at each plate equilibrium of the analyte is established between mobile and stationary phase. Movement of analyte and mobile phase is viewed as a series of transfers from one plate to the next. Efficiency of a column

One goal of chromatography is to achieve sharp symmetrical peaks, thus optimizing analyte separation and improving detection. The sharpness of the peak represents the efficiency of the chromatographic column (actually the entire system). Two general approaches have been developed to measure column efficiency – plate theory and rate theory.