2019年2月28日 · A transgenic mouse containing a CRE-recombinase inducible CAG promoter driven CD9 protein fused to Turbo-GFP derived from the copepod Pontellina plumata was generated as an EV reporter.

TSPAN4 or CD9 with a green fluorescent protein (GFP) reporter attached to the C terminus of TSPAN proteins was transiently expressed in HEK293T cells (Fig. 1A; see Materials and Methods for details). GPMVs with TSPAN4 or CD9 in their membrane were immobilized on a glass surface within a custom-made chamber mounted on the stage of a correlated ...

2022年1月11日 · We, therefore, created an EV reporter using truncated CD9 to display enhanced green fluorescent protein (EGFP) on the EV surface. CD9truc-EGFP expression in cells did not affect EV size and...

Plasmid pLenti6.3/TO/CD9-GFP from Dr. Jens Gruber's lab contains the insert CD9 and is published in Mol Ther. 2018 Feb 7;26(2):634-647. doi: 10.1016/j.ymthe.2017.11.008. Epub 2017 Nov 16. This plasmid is available through Addgene.

033361 B6;129S1- Gt(ROSA)26Sor tm1(CAG-CD9/GFP)Dmfel /J TIGER knock-in mice ( t ransgenic i nducible G FP E V r eporter) express a Cre-inducible His-tagged CD9/TurboGFP reporter targeted to extracellular vesicles. These mice allow in vivo imaging of extracellular vesicles and are suitable for use in applications related to the study of ...

2019年2月28日 · Among the most plentiful and frequently identified EV proteins is CD9, a tetraspanin protein. A transgenic mouse containing a CRE-recombinase inducible CAG promoter driven CD9 protein fused to Turbo-GFP derived from the copepod Pontellina plumata was generated as an EV reporter.

2020年3月30日 · GFP-labeled CD9 and EWI-2 were co-expressed in HEK cells, and complex formation was analyzed by FSEC. The slight peak shift toward the smaller molecular weight indicates the complex formation...

2025年3月28日 · EVs were isolated from CD81-RFP and CD9-GFP co-transfected HEK293T cells using ultracentrifugation, as described in ‘Preparation and purification of AP-EVs’. The EV suspension was passed through a 0.22-µm filter and diluted to 2 × 10 8 particles/30 µL PBS. A 30-µL suspension was applied onto a glass slide and covered with a cover slip.

2019年2月28日 · CD9-GFP labeled cells included glutamine synthetase and glial fibrillary acidic protein positive astrocytes. Cortical astrocytes released ~136 nm EVs that contained CD9. Intraventricular injected EVs were taken up by CD11b/IBA1 positive microglia surrounding the lateral ventricles.

2018年6月15日 · Here, as a proof of principle, we demonstrate that CD9 GFP overexpression (1) boosts exosome release of AAV producer cells, (2) promotes the production of more efficient exo-AAVs, and (3) increases the amount of exosome-associated AAVs.