T cell stimulation of B cell proliferation during T-B collaboration requires membrane-bound stimulatory ligands, such as CD40 ligand and the secretion of soluble cytokines, such as IL-4. Nevertheless, it remains unclear whether T cell contact is …

IL-4 促进 B 细胞 MHCⅡ类抗原、FcεRⅡ/CD23 和 CD40 的表达，并增强 B 细胞提呈抗原能力，使免疫系统对小量抗原刺激发生免疫应答。 增加 FcεRⅡ/CD23 (IgE Fc 段低亲和力受体) 的表达，并释放可溶性 CD23 (sCD23)/IgE 结合因子 (IgE-BF)，与 mIgE 阳性细胞结合并诱导其分化，可能与促进B细胞 IgE 的产生有关。 IL-4 提高 LPS 刺激小鼠 B 细胞 IgG1、IgE 产生水平分别为 8～10 倍和 10～100 倍，但对 IgG3 分泌降低 6～10 倍，IgG2a、IgG2b 和 IgM 有不同程度下 …

B 淋巴细胞具有多种对体液免疫至关重要的功能，包括向 T 细胞呈递抗原、分泌细胞因子和分化为分泌抗体的浆细胞。 通过持续 IL-4 刺激和 CD40L 结合其 CD40 受体来体外扩增人类 B 细胞，使得这些 IL-4-CD40-B 细胞能够用于诱导 Ag 特异性 T 细胞免疫反应的研究。

2021年7月1日 · In this report, we characterized B cells stimulated with CD40L and the combination of IL-4 and IL-21 (Combo-CD40–B cells). We demonstrate that Combo-CD40–B cells are mostly activated mature B cell subsets that secrete cytokines/chemokines and express molecules associated with strong APC functions.

背景:B细胞活化,增殖和分化为抗体产生细胞是机体体液免疫应答的主要过程,在疾病防御中发挥十分重要的作用.然而,针对自身抗原和过敏原的B细胞的过度激活会导致自身免疫性疾病和过敏性疾病.目的 :本研究将通过优化诱导B细胞活化,增殖和分化的条件,为B细胞 ...

2021年7月1日 · B lymphocytes have multiple functions central to humoral immunity, including Ag presentation to T cells, cytokine secretion, and differentiation into Ab-secreting plasma cells. In vitro expansion of human B cells by continuous IL-4 stimulation and engagement of their CD40 receptor by CD40L has allow …

2024年3月26日 · CD40是一种重要的免疫调节分子,属于肿瘤坏死因子受体超家族成员。 它主要表达在抗原提呈细胞 (如 B细胞 、树突状细胞和巨噬细胞)表面,而其配体 CD40L 则表达在活化的 T细胞 表面。

2020年2月13日 · Herein, we report that the combination of agonistic anti-CD40, IL-4 and IL-21 affords polyclonal B cell stimulation that was comparable to R848 and IL-2 for detection of influenza-specific memory B cells.

2007年5月1日 · We sought to discover the mechanisms explaining increased IgE production seen at low cell densities when IL-21 is added to human B cell cultures activated with anti-CD40 and IL-4. When cells were cultured in the absence of BCR ligation, qPCR demonstrated dramatic increases in mRNA for the plasma cell transcription factors BLIMP1 and XBP1.

1991年7月1日 · IL-4 induces IgE and IgG4 synthesis, but in addition to IL-4, a second signal provided by CD4+ T cells is required. Here we demonstrate that the signal provided by CD4+ T cells can be replaced by anti-CD40 mAb.