2023年7月25日 · CRISPR/Cas9系统是一种多功能的工具，可以在许多生物体和模型系统中进行精确的基因编辑。 研究人员已经广泛使用CRISPR/Cas9对人诱导性多能干细胞(iPSCs)进行序列特异性改变。

2024年9月27日 · CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating precise site-specific DNA insertions, deletions, and substitutions. These precise edits are made possible through the use of exogenous donor templates that …

2018年5月31日 · We present a strategy to enhance HDR efficiency by covalently tethering a single-stranded oligodeoxynucleotide (ssODN) to the Cas9-guide RNA ribonucleoprotein (RNP) complex via a fused HUH...

2024年12月10日 · Here, we review CRISPR-Cas9-mediated HDR, with a focus on methodologies for boosting HDR efficiency, and applications of precise editing via HDR. First, we describe two common mechanisms of DNA repair, non-homologous end joining (NHEJ), and HDR, and discuss their impact on CRISPR-Cas9-mediated precise genome editing.

2024年8月10日 · The HDR-boosting modules designed from the binding ODNs of the human RAD51 protein enhanced HDR efficiency mediated by Cas9 across multiple genomic loci and various cell types.

2020年5月19日 · 通过 CRISPR-Cas9 生成双链断裂后，哺乳动物细胞会通过内源性细胞机制修复断裂的位点，典型的机制包括非同源末端连接（NHEJ）通路和同源介导修复（HDR）通路 [1]。 这正是 CRISPR-Cas9 介导基因编辑的原理，当 CRISPR 体系切割产生双链断裂后（double-stranded break），细胞内 DNA 修复遵循以下两种机制： 一是非同源末端连接（non-homologous end joining，NHEJ），断裂 DNA 直接连接；另一种是同源定向修复 （homology-directed …

2025年3月5日 · In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which enhances the efficiency of targeted gene insertion via the HDR pathway.

2021年9月30日 · Here, we describe comprehensive design considerations and optimized methods for highly efficient HDR using single-stranded oligodeoxynucleotide (ssODN) donor templates for several CRISPR–Cas...

CRISPR/Cas9基因编辑利用Cas9核酸酶及伴随的小向导RNA（sgRNA）定点产生DNA双链断裂（DNA double strand break, DSB），依靠细胞自身的DSB修复机制完成修复，产生包含编辑产物的修复产物[1]。

2024年9月26日 · Here, we review CRISPR-Cas9-mediated HDR, with a focus on methodologies for boosting HDR efficiency, and applications of precise editing via HDR. First, we describe two common mechanisms of DNA repair, non-homologous end joining (NHEJ), and HDR, and discuss their impact on CRISPR-Cas9-mediated precise genome editing.