Recombinant Cas9-GFP protein from Streptococcus pyogenes (~194 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide …

Select from Cas9 nucleases with improved editing efficiency, increased specificity, and GFP/RFP tags for visualization, always synthesized to our high quality and purity standards.

Plasmid pLenti-Cas9-GFP from Dr. David Sabatini's lab contains the insert Cas9-GFP and is published in Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. …

Plasmid pSpCas9(BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub …

wild type Streptococcus pyogenes Cas91 (Cas9-GFP Protein) or enhanced specificity Cas92 (eSpCas9-GFP Protein). Both proteins contain three varied nuclear localization sequences …

Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

Compared with the ectopically expressed GFP-LC3, the endogenous knock-in reporter exhibited much higher sensitivity and signal-to-noise ratio of GFP-LC3 puncta upon the induction or …

In this protocol, Sp Cas9 is expressed as a protein fused to green fluorescent protein (GFP), and maltose binding protein (MBP). The GFP tag allows for the presence of the recombinant …

By directly delivering Cas9/sgRNA ribonucleoprotein (RNP) complexes through nucleofection, this protocol allows for the efficient integration of GFP 11 into a specific genomic locus via CRISPR …

CRISPR/Cas9 Lenti-ONE vectors allows to easily and efficiently deliver the CRISPR system (“Clustered Regularly Interspaced Short Palindromic Repeats”) into desired in vivo/in vitro cell …